Detailed information of TA system TA241966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5089490..5090160
• Replicon: chromosome
• Accession: NZ_CP095426
• Organism: Klebsiella pneumoniae strain IM029

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MWR69_RS25260
• Protein ID: WP_004213072.1
• Coordinates: 5089490..5089933 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MWR69_RS25265
• Protein ID: WP_004213073.1
• Coordinates: 5089930..5090160 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR69_RS25225 (MWR69_25195)	5084911..5085177	+	267	WP_147021650.1	carboxymuconolactone decarboxylase family protein	-
MWR69_RS25230 (MWR69_25200)	5085240..5085731	+	492	WP_011251327.1	DM13 domain-containing protein	-
MWR69_RS25235 (MWR69_25205)	5085780..5086700	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
MWR69_RS25240 (MWR69_25210)	5086791..5087194	+	404	Protein_4952	GAF domain-containing protein	-
MWR69_RS25245 (MWR69_25215)	5087712..5088346	-	635	Protein_4953	mucoid phenotype regulator RmpA2	-
MWR69_RS25250 (MWR69_25220)	5088763..5089067	+	305	Protein_4954	transposase	-
MWR69_RS25255 (MWR69_25225)	5089090..5089341	-	252	WP_186987481.1	hypothetical protein	-
MWR69_RS25260 (MWR69_25230)	5089490..5089933	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MWR69_RS25265 (MWR69_25235)	5089930..5090160	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MWR69_RS25270 (MWR69_25240)	5090768..5091901	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MWR69_RS25275 (MWR69_25245)	5091917..5092210	+	294	WP_004213076.1	hypothetical protein	-
MWR69_RS25280 (MWR69_25250)	5092200..5092406	-	207	WP_004213077.1	hypothetical protein	-
MWR69_RS25285 (MWR69_25255)	5092758..5093048	+	291	WP_004213078.1	hypothetical protein	-
MWR69_RS25290 (MWR69_25260)	5093038..5093937	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / qnrB4 / blaDHA-1 / mph(A) / blaTEM-1B / blaCTX-M-15 / aac(6')-Ib-cr / blaOXA-1 / tet(A) / blaSHV-187 / aph(3')-Ia	iucA / iucB / iucC / iucD / iutA / rmpA	4994180..5228537	234357

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T241966 WP_004213072.1 NZ_CP095426:c5089933-5089490 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T241966 NZ_OU701452:4901917-4902207 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT241966 WP_004213073.1 NZ_CP095426:c5090160-5089930 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT241966 NZ_OU701452:4901685-4901927 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620