Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4173827..4174446 Replicon chromosome
Accession NZ_CP095426
Organism Klebsiella pneumoniae strain IM029

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MWR69_RS20815 Protein ID WP_002892050.1
Coordinates 4174228..4174446 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MWR69_RS20810 Protein ID WP_002892066.1
Coordinates 4173827..4174201 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR69_RS20800 (MWR69_20775) 4168979..4170172 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWR69_RS20805 (MWR69_20780) 4170195..4173341 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MWR69_RS20810 (MWR69_20785) 4173827..4174201 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MWR69_RS20815 (MWR69_20790) 4174228..4174446 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MWR69_RS20820 (MWR69_20795) 4174605..4175171 + 567 WP_002892030.1 maltose O-acetyltransferase -
MWR69_RS20825 (MWR69_20800) 4175143..4175283 - 141 WP_004147370.1 hypothetical protein -
MWR69_RS20830 (MWR69_20805) 4175304..4175774 + 471 WP_002892026.1 YlaC family protein -
MWR69_RS20835 (MWR69_20810) 4175749..4177200 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MWR69_RS20840 (MWR69_20815) 4177301..4177999 + 699 WP_002892021.1 GNAT family protein -
MWR69_RS20845 (MWR69_20820) 4177996..4178136 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MWR69_RS20850 (MWR69_20825) 4178136..4178399 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241962 WP_002892050.1 NZ_CP095426:4174228-4174446 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T241962 NZ_OU701452:c4130803-4130618 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT241962 WP_002892066.1 NZ_CP095426:4173827-4174201 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT241962 NZ_OU701452:c4130601-4130191 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References