Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 172683..173419 Replicon plasmid pIM057_vir
Accession NZ_CP095422
Organism Klebsiella pneumoniae strain IM057

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag MWR70_RS28195 Protein ID WP_004098919.1
Coordinates 172683..173165 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag MWR70_RS28200 Protein ID WP_004213599.1
Coordinates 173153..173419 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR70_RS28175 (MWR70_28135) 168037..169383 + 1347 WP_011154555.1 dihydroorotase -
MWR70_RS28180 (MWR70_28140) 169447..170469 + 1023 WP_004214536.1 porphobilinogen synthase -
MWR70_RS28185 (MWR70_28145) 170644..171039 + 396 Protein_197 IS3 family transposase -
MWR70_RS28190 (MWR70_28150) 171035..172262 - 1228 Protein_198 IS3 family transposase -
MWR70_RS28195 (MWR70_28155) 172683..173165 - 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
MWR70_RS28200 (MWR70_28160) 173153..173419 - 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
MWR70_RS28205 (MWR70_28165) 173614..173844 - 231 WP_004213598.1 hypothetical protein -
MWR70_RS28210 (MWR70_28170) 173858..174061 - 204 WP_004213596.1 HHA domain-containing protein -
MWR70_RS28215 (MWR70_28175) 174122..174616 - 495 WP_004213594.1 hypothetical protein -
MWR70_RS28220 (MWR70_28180) 174902..175906 - 1005 WP_000427619.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 / rmpA 1..208817 208817
- inside IScluster/Tn - - 171035..179514 8479


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T241939 WP_004098919.1 NZ_CP095422:c173165-172683 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T241939 NZ_OU701449:c4966730-4966419 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT241939 WP_004213599.1 NZ_CP095422:c173419-173153 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT241939 NZ_OU701449:c4966418-4966128 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References