Detailed information of TA system TA241938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 30691..31361
• Replicon: plasmid pIM057_vir
• Accession: NZ_CP095422
• Organism: Klebsiella pneumoniae strain IM057

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MWR70_RS27380
• Protein ID: WP_004213072.1
• Coordinates: 30691..31134 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MWR70_RS27385
• Protein ID: WP_004213073.1
• Coordinates: 31131..31361 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWR70_RS27345 (MWR70_27315)	26093..26368	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
MWR70_RS27350 (MWR70_27320)	26431..26922	+	492	WP_011251327.1	DM13 domain-containing protein	-
MWR70_RS27355 (MWR70_27325)	26971..27891	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
MWR70_RS27360 (MWR70_27330)	27982..28385	+	404	Protein_32	GAF domain-containing protein	-
MWR70_RS27365 (MWR70_27335)	28904..29547	-	644	Protein_33	mucoid phenotype regulator RmpA2	-
MWR70_RS27370 (MWR70_27340)	29964..30268	+	305	Protein_34	transposase	-
MWR70_RS27375 (MWR70_27345)	30291..30542	-	252	WP_186987481.1	hypothetical protein	-
MWR70_RS27380 (MWR70_27350)	30691..31134	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MWR70_RS27385 (MWR70_27355)	31131..31361	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MWR70_RS27390 (MWR70_27360)	31969..33102	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MWR70_RS27395 (MWR70_27365)	33118..33411	+	294	WP_004213076.1	hypothetical protein	-
MWR70_RS27400 (MWR70_27370)	33401..33607	-	207	WP_004213077.1	hypothetical protein	-
MWR70_RS27405 (MWR70_27375)	33959..34249	+	291	WP_004213078.1	hypothetical protein	-
MWR70_RS27410 (MWR70_27380)	34239..35138	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA / rmpA2 / rmpA	1..208817	208817

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T241938 WP_004213072.1 NZ_CP095422:c31134-30691 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T241938 NZ_OU701449:c4669835-4669662 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT241938 WP_004213073.1 NZ_CP095422:c31361-31131 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT241938 NZ_OU701449:c4670159-4669863 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620