Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5424311..5424936 Replicon chromosome
Accession NZ_CP095421
Organism Klebsiella pneumoniae strain IM057

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MWR70_RS26835 Protein ID WP_002882817.1
Coordinates 5424311..5424694 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MWR70_RS26840 Protein ID WP_004150355.1
Coordinates 5424694..5424936 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR70_RS26820 (MWR70_26790) 5421677..5422579 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MWR70_RS26825 (MWR70_26795) 5422576..5423211 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MWR70_RS26830 (MWR70_26800) 5423208..5424137 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MWR70_RS26835 (MWR70_26805) 5424311..5424694 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MWR70_RS26840 (MWR70_26810) 5424694..5424936 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MWR70_RS26845 (MWR70_26815) 5425141..5426058 + 918 WP_002882812.1 alpha/beta hydrolase -
MWR70_RS26850 (MWR70_26820) 5426072..5427013 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MWR70_RS26855 (MWR70_26825) 5427058..5427495 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MWR70_RS26860 (MWR70_26830) 5427492..5428352 - 861 WP_002882807.1 virulence factor BrkB family protein -
MWR70_RS26865 (MWR70_26835) 5428346..5428945 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241937 WP_002882817.1 NZ_CP095421:c5424694-5424311 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241937 NZ_OU701449:c4572875-4572525 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241937 WP_004150355.1 NZ_CP095421:c5424936-5424694 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241937 NZ_OU701449:c4573120-4572869 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References