Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4225827..4226446 Replicon chromosome
Accession NZ_CP095421
Organism Klebsiella pneumoniae strain IM057

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MWR70_RS21080 Protein ID WP_002892050.1
Coordinates 4226228..4226446 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MWR70_RS21075 Protein ID WP_002892066.1
Coordinates 4225827..4226201 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWR70_RS21065 (MWR70_21040) 4220979..4222172 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWR70_RS21070 (MWR70_21045) 4222195..4225341 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MWR70_RS21075 (MWR70_21050) 4225827..4226201 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MWR70_RS21080 (MWR70_21055) 4226228..4226446 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MWR70_RS21085 (MWR70_21060) 4226605..4227171 + 567 WP_002892030.1 maltose O-acetyltransferase -
MWR70_RS21090 (MWR70_21065) 4227143..4227283 - 141 WP_004147370.1 hypothetical protein -
MWR70_RS21095 (MWR70_21070) 4227304..4227774 + 471 WP_002892026.1 YlaC family protein -
MWR70_RS21100 (MWR70_21075) 4227749..4229200 - 1452 WP_271229871.1 PLP-dependent aminotransferase family protein -
MWR70_RS21105 (MWR70_21080) 4229301..4229999 + 699 WP_002892021.1 GNAT family protein -
MWR70_RS21110 (MWR70_21085) 4229996..4230136 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MWR70_RS21115 (MWR70_21090) 4230136..4230399 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241933 WP_002892050.1 NZ_CP095421:4226228-4226446 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T241933 NZ_OU701449:3782918-3783136 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT241933 WP_002892066.1 NZ_CP095421:4225827-4226201 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT241933 NZ_OU701449:3782518-3782892 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References