Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 8401..8929 Replicon plasmid pTCM-2
Accession NZ_CP095409
Organism Acinetobacter pittii strain TCM

Toxin (Protein)


Gene name relE Uniprot ID N9N871
Locus tag MWH18_RS21565 Protein ID WP_000221358.1
Coordinates 8636..8929 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID N9NWT9
Locus tag MWH18_RS21560 Protein ID WP_000246755.1
Coordinates 8401..8646 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWH18_RS21530 (MWH18_21525) 3676..3993 - 318 WP_005065053.1 hypothetical protein -
MWH18_RS21535 (MWH18_21530) 4331..4855 - 525 WP_005127114.1 plasmid replication DNA-binding protein -
MWH18_RS21540 (MWH18_21535) 4934..5863 - 930 WP_005133531.1 replication initiation protein RepM -
MWH18_RS21545 (MWH18_21540) 7270..7461 + 192 WP_000102616.1 hypothetical protein -
MWH18_RS21550 (MWH18_21545) 7504..7902 - 399 WP_024437500.1 hypothetical protein -
MWH18_RS21555 (MWH18_21550) 7924..8259 - 336 WP_159452829.1 hypothetical protein -
MWH18_RS21560 (MWH18_21555) 8401..8646 + 246 WP_000246755.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MWH18_RS21565 (MWH18_21560) 8636..8929 + 294 WP_000221358.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWH18_RS21570 (MWH18_21565) 9169..9615 + 447 WP_006581726.1 GNAT family N-acetyltransferase -
MWH18_RS21575 (MWH18_21570) 9849..10160 + 312 WP_005086328.1 BrnT family toxin -
MWH18_RS21580 (MWH18_21575) 10123..10437 + 315 WP_005086327.1 BrnA antitoxin family protein -
MWH18_RS21585 (MWH18_21580) 10532..11059 + 528 WP_000537572.1 plasmid pRiA4b ORF-3 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11346 11346


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11292.52 Da        Isoelectric Point: 10.4753

>T241914 WP_000221358.1 NZ_CP095409:8636-8929 [Acinetobacter pittii]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Download         Length: 294 bp

>T241914 NZ_OU659204:c3850364-3850062 [Providencia alcalifaciens]
ATGTTGACCGTACTAACGACAGATATCTTTAATAGATGGATGCATGACCTGAAAGATATTCGAGCAAAAACAAAGATTCA
GGTAAGAATTCGGCGTTTGAAACAAGGCAATTTTGGTGATGTCGAACCGATAGGTGATGGTTTTTCTGAATTAAAAATAC
ATGAAGGGAAAGGATATCGCGTTTATTTAAGAAAGATTGATAATACAATCGTATTGTTAATTAGTGGTGGTGATAAATCA
ACGCAGCAAAAAGATATCGACAAAGCCAAACAAATTTTTCGTGAAATTGAAGGTGATCTATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9249.64 Da        Isoelectric Point: 4.3142

>AT241914 WP_000246755.1 NZ_CP095409:8401-8646 [Acinetobacter pittii]
MATVNFRIDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Download         Length: 246 bp

>AT241914 NZ_OU659204:c3850065-3849778 [Providencia alcalifaciens]
ATGAGTATTAAAATAAAAGAGTATGACATTGCTGAGCATTTAACGAACGAAGAAGAAATTCAGCTGTATCTTAATGAAAT
ACTAGAAGAAGGCGATACGGCACTCATTCTTGCTGCATTAGGCGATATTGCGAGAGCGAGAAATATGACCCAATTAGCAA
AGGAAGTGGGCATGAGCCGAGAAGGGCTATATAAGGCACTATCTGGTGAAGGTAACCCTGCATTTACCACTATCTTGAAG
ATAGTTCATTCACTCGGACTAAAGCTGCAATTTAAGAGCGCAAGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A1RKP4

Antitoxin

Source ID Structure
AlphaFold DB A0A6A1RJ80

References