Detailed information of TA system TA024191

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2152877..2153348
• Replicon: chromosome
• Accession: NC_013008
• Organism: Escherichia coli O157:H7 str. TW14359

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: ECSP_RS11125
• Protein ID: WP_001303511.1
• Coordinates: 2153070..2153348 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: ECSP_RS11120
• Protein ID: WP_001302048.1
• Coordinates: 2152877..2153068 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECSP_RS11075	2148084..2148668	-	585	WP_000206793.1	DUF551 domain-containing protein	-
ECSP_RS11080	2148725..2149120	-	396	WP_001118159.1	DUF977 family protein	-
ECSP_RS11085	2149136..2149905	-	770	Protein_2100	DUF1627 domain-containing protein	-
ECSP_RS11095	2149931..2150671	-	741	WP_000788938.1	ATP-binding protein	-
ECSP_RS11100	2150678..2151640	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
ECSP_RS11105	2151663..2152088	-	426	WP_000693943.1	Rha family transcriptional regulator	-
ECSP_RS11110	2152085..2152387	-	303	WP_000172738.1	transcriptional regulator	-
ECSP_RS32140	2152485..2152856	+	372	WP_001169686.1	hypothetical protein	-
ECSP_RS11120	2152877..2153068	+	192	WP_001302048.1	hypothetical protein	Antitoxin
ECSP_RS11125	2153070..2153348	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECSP_RS11135	2153701..2154000	+	300	WP_001240334.1	hypothetical protein	-
ECSP_RS11140	2154072..2154290	+	219	WP_001171930.1	hypothetical protein	-
ECSP_RS30290	2154859..2155047	+	189	WP_000449192.1	cell division inhibition protein DicB	-
ECSP_RS11145	2155044..2155235	+	192	WP_001090200.1	DUF1482 family protein	-
ECSP_RS11150	2155328..2156413	+	1086	Protein_2112	exonuclease	-
ECSP_RS11160	2156464..2157677	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	2070475..2162948	92473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T24191 WP_001303511.1 NC_013008:2153070-2153348 [Escherichia coli O157:H7 str. TW14359]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T24191 NC_013008:2153070-2153348 [Escherichia coli O157:H7 str. TW14359]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT24191 WP_001302048.1 NC_013008:2152877-2153068 [Escherichia coli O157:H7 str. TW14359]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT24191 NC_013008:2152877-2153068 [Escherichia coli O157:H7 str. TW14359]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0