Detailed information of TA system TA241907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higB-toxA/Tad-HTH_37
• Location: 2293226..2293854
• Replicon: chromosome
• Accession: NZ_CP095406
• Organism: Lacticaseibacillus paracasei strain GM-080

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A806L3G4
• Locus tag: MWH19_RS11205
• Protein ID: WP_012490993.1
• Coordinates: 2293486..2293854 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: toxA
• Uniprot ID: -
• Locus tag: MWH19_RS11200
• Protein ID: WP_003583264.1
• Coordinates: 2293226..2293486 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWH19_RS11175 (MWH19_11155)	2289117..2289860	+	744	WP_012490990.1	SDR family oxidoreductase	-
MWH19_RS11180 (MWH19_11160)	2289973..2290599	+	627	WP_003577713.1	flavodoxin	-
MWH19_RS11185 (MWH19_11165)	2290850..2291353	+	504	WP_012490991.1	TetR/AcrR family transcriptional regulator	-
MWH19_RS11190 (MWH19_11170)	2291358..2292419	+	1062	WP_003563374.1	ABC transporter permease	-
MWH19_RS11195 (MWH19_11175)	2292424..2293113	+	690	WP_012490992.1	ABC transporter ATP-binding protein	-
MWH19_RS11200 (MWH19_11180)	2293226..2293486	-	261	WP_003583264.1	helix-turn-helix transcriptional regulator	Antitoxin
MWH19_RS11205 (MWH19_11185)	2293486..2293854	-	369	WP_012490993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MWH19_RS11210 (MWH19_11190)	2294145..2295515	-	1371	WP_012490994.1	FAD-dependent oxidoreductase	-
MWH19_RS11215 (MWH19_11195)	2295879..2296697	-	819	WP_012490995.1	Cof-type HAD-IIB family hydrolase	-
MWH19_RS11220 (MWH19_11200)	2297013..2297282	-	270	WP_012490996.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
MWH19_RS11225 (MWH19_11205)	2297523..2298530	+	1008	WP_003563383.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (34-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-79)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 14970.02 Da
• Isoelectric Point: 8.3986

>T241907 WP_012490993.1 NZ_CP095406:c2293854-2293486 [Lacticaseibacillus paracasei]
MYKIDFYEDRDGYSEIEDFLDQLRHSHQKNNVSLLNKITKELYMLQNLGPRLREPHAKFLKGYAYPIMELRPMQERIFYA
AWQKDHFVLLHHYTKKTNKTDHREILRAQSNLEDWLSRKEEN

Nucleotide

• Length: 369 bp

>T241907 NZ_OU659204:c1449429-1449121 [Providencia alcalifaciens]
ATGCAATATTGTGTCTATCAAGCTAAAAAAGAGCCAACCAACTATCCTTATCTTTTGGATGTACAAAGTGACATTATTGA
TGACTTAACAACACGCCTTGTCATTCCTTTATTTGATACACGTCATCTGCAAGCTGCCCTGCCGATTCGACTTAACCCAA
TGATTGATATTGAAGGGCAACCCTTTGTTCTTATGACTCATTTGATGTCAGCGATCTCTAAATCAATGCTAGGAAAAGAG
GTTATTTGCATCGAGCACCAAAGAGATAAAATCAAAAGTGCAATCGACCTGCTATTTGATGGCTTTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9828.45 Da
• Isoelectric Point: 10.2439

>AT241907 WP_003583264.1 NZ_CP095406:c2293486-2293226 [Lacticaseibacillus paracasei]
MTTWEDFSQKLKKDNPEKLAIIKTLADLESERIKQGISQKDFAEKIGMKQPQLAKIERLDSLPTLATLNRYARGLGKEIR
LSIVTQ

Nucleotide

• Length: 261 bp

>AT241907 NZ_OU659204:c1449665-1449429 [Providencia alcalifaciens]
ATGACGGTAGCCAATAAAATGAAAAAGACGGTGAGTGTGACAGTCTCCCCTGAACTTTATGAACAAGCGAGAAAAGCCAA
TTTGAATTTCTCCCTCGTGTTATCCAATGCTTTACTGGCTGAACTCAAAAAACTTGAGGCGTTAAAATGGAAAGCGGATA
ACCAAGATGGTTTTCAGGAACTCAATAGGATCAGTGAAGAATATGGAATTTTTTCAGAGGAATATAAGGAGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A806L3G4