241906

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1431681..1432332 Replicon chromosome
Accession NZ_CP095406
Organism Lacticaseibacillus paracasei strain GM-080

Toxin (Protein)


Gene name pemK Uniprot ID K6S8L5
Locus tag MWH19_RS06965 Protein ID WP_003567661.1
Coordinates 1431681..1432064 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID K6RKI5
Locus tag MWH19_RS06970 Protein ID WP_003567665.1
Coordinates 1432084..1432332 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWH19_RS06960 (MWH19_06945) 1430986..1431507 - 522 WP_012492007.1 QueT transporter family protein -
MWH19_RS06965 (MWH19_06950) 1431681..1432064 - 384 WP_003567661.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
MWH19_RS06970 (MWH19_06955) 1432084..1432332 - 249 WP_003567665.1 antitoxin Antitoxin
MWH19_RS06975 (MWH19_06960) 1432400..1433536 - 1137 WP_003576478.1 alanine racemase -
MWH19_RS06980 (MWH19_06965) 1433523..1433897 - 375 WP_283544575.1 holo-ACP synthase -
MWH19_RS06985 (MWH19_06970) 1434067..1435575 - 1509 WP_003567670.1 DEAD/DEAH box helicase -
MWH19_RS06990 (MWH19_06975) 1435701..1435844 - 144 WP_003588978.1 hypothetical protein -
MWH19_RS06995 (MWH19_06980) 1435875..1437263 - 1389 WP_003567677.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13773.03 Da        Isoelectric Point: 10.9764

>T241906 WP_003567661.1 NZ_CP095406:c1432064-1431681 [Lacticaseibacillus paracasei]
MDVSVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSTKMAAVDRALAISVGLVSLPKPKTYNKN

Download         Length: 384 bp

>T241906 NZ_OU659204:c834263-834060 [Providencia alcalifaciens]
ATGACCAAATCCGATTATCTGATGCGTTTAAGAAAATGCACCACTATTGAAACACTTGAGCGCGTCATTGAGAAAAATAA
GTATGAGTTATCTGATGATGAATTAGAGTTATTTTACTCTGCAGCTGATCATCGTCTTGCCGAACTCACTATGAATAAAC
TTTACGATAAAATCCCAGCATCTGTTTGGAAGTTTGTTAGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8938.08 Da        Isoelectric Point: 4.4610

>AT241906 WP_003567665.1 NZ_CP095406:c1432332-1432084 [Lacticaseibacillus paracasei]
MKKQTSVAVTFDDAIKARLDAYCELNDIDAQAVITEAVATFLKSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Download         Length: 249 bp

>AT241906 NZ_OU659204:c834704-834336 [Providencia alcalifaciens]
ATGGATGAATACTCACCCAAGAAACATGATATAGCCGAATTAAAATATCTGTGTAACAGTTTGAATAGAGACGCAATATC
AAGTTTACAAAAAACAAACACCCATTGGATAAATGATCTTAGTTCGGCACAAAGCATCAGCCTCAATGAGCTCGTTGAGC
ATATTGCAGCTTTTGTCTGGCGATTCAAAATCAAGTATCCAAAAGAAAATCTCGTTATTTCTCTTGTTGAAGAGTATCTT
GACGAGACATATAATTTATTTGGCAGCCCAGTAATTACTTTCAACGAAATCATTGACTGGGAAAGTATGAATCAAAATCT
TGTCGCCGTGCTCGATGATGATCTAAAATGCCTGACAAGTAAGACATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K6S8L5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BNY2

References