Detailed information of TA system TA241902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2490116..2490767
• Replicon: chromosome
• Accession: NZ_CP095384
• Organism: Lacticaseibacillus rhamnosus strain VHProbi M14

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: K8Q4Y0
• Locus tag: MU539_RS11660
• Protein ID: WP_005686631.1
• Coordinates: 2490116..2490499 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: K8QH19
• Locus tag: MU539_RS11665
• Protein ID: WP_005686632.1
• Coordinates: 2490519..2490767 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU539_RS11655 (MU539_11630)	2489076..2489603	-	528	WP_033571829.1	QueT transporter family protein	-
MU539_RS11660 (MU539_11635)	2490116..2490499	-	384	WP_005686631.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MU539_RS11665 (MU539_11640)	2490519..2490767	-	249	WP_005686632.1	antitoxin	Antitoxin
MU539_RS11670 (MU539_11645)	2490879..2492018	-	1140	WP_033571831.1	alanine racemase	-
MU539_RS11675 (MU539_11650)	2492005..2492379	-	375	WP_033571832.1	holo-ACP synthase	-
MU539_RS11680 (MU539_11655)	2492548..2494056	-	1509	WP_005686635.1	DEAD/DEAH box helicase	-
MU539_RS11685 (MU539_11660)	2494330..2495718	-	1389	WP_033571853.1	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-115)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13757.03 Da
• Isoelectric Point: 10.9764

>T241902 WP_005686631.1 NZ_CP095384:c2490499-2490116 [Lacticaseibacillus rhamnosus]
MDVTVKRGDVFFADLSPVVGSEQGGNRPVLIIQNNVGNHYSPTVIVAAITSKIQKPKMPTHVGLRAKQDGVERNSVILLE
QIRTIDKQRLQARVTALSSAKMAAVDRALAISVGLVSLPKPKTYNKN

Nucleotide

• Length: 384 bp

>T241902 NZ_OU659164:44970-45248 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATGCCACACGAAGTCCGTCGGATTGTGGTGGTGAATTATGAGATACGTTATGAACTCATTGATTCGACAATC
TATATTTTACGGATATGGCATACACGAGAAGATCGGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8936.13 Da
• Isoelectric Point: 4.4778

>AT241902 WP_005686632.1 NZ_CP095384:c2490767-2490519 [Lacticaseibacillus rhamnosus]
MKKETSVTVALDSAIKARLDAYCELNDMGVQDVITEAVAKFLQSQAPEINQLISGYVAMGQINTEISHAFSACESEAYAH
IR

Nucleotide

• Length: 249 bp

>AT241902 NZ_OU659164:45248-45391 [Providencia alcalifaciens]
ATGAGACAATTTAAAGTATCTCACCTTGGTGAAATTATCGCTAGAGACTTGGCTGATATGGGGATTAGTGGTCGACGTTT
CGCGCTCAATATTGATGTCACTCCAGCGACCGCTGGCAATTCGAATTGCTGCTGCATTAGATAG

Structures

Toxin

• AlphaFold DB: K8Q4Y0

Antitoxin

• AlphaFold DB: K8QH19