Detailed information of TA system TA241900

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2274049..2275027
• Replicon: chromosome
• Accession: NZ_CP095377
• Organism: Bacillus cereus strain SEM-15

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: MWG54_RS11770
• Protein ID: WP_000624977.1
• Coordinates: 2274049..2274786 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: MWG54_RS11775
• Protein ID: WP_000237818.1
• Coordinates: 2274899..2275027 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWG54_RS11745 (MWG54_11745)	2269665..2270072	+	408	WP_000063589.1	VOC family protein	-
MWG54_RS11750 (MWG54_11750)	2270085..2270474	+	390	Protein_2235	SAM-dependent methyltransferase	-
MWG54_RS11755 (MWG54_11755)	2270632..2272317	-	1686	WP_246962751.1	alpha-keto acid decarboxylase family protein	-
MWG54_RS11760 (MWG54_11760)	2272425..2272907	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
MWG54_RS11765 (MWG54_11765)	2273074..2273811	+	738	WP_246962753.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
MWG54_RS11770 (MWG54_11770)	2274049..2274786	+	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
MWG54_RS11775 (MWG54_11775)	2274899..2275027	+	129	WP_000237818.1	hypothetical protein	Antitoxin
MWG54_RS11780 (MWG54_11780)	2275100..2275276	+	177	WP_000852629.1	stage II sporulation protein SB	-
MWG54_RS11785 (MWG54_11785)	2275294..2275683	-	390	WP_000713867.1	YxeA family protein	-
MWG54_RS11790 (MWG54_11790)	2275895..2277364	+	1470	WP_246962755.1	beta-Ala-His dipeptidase	-
MWG54_RS11795 (MWG54_11795)	2277610..2278419	+	810	WP_246962757.1	papain-like cysteine protease family protein	-
MWG54_RS11800 (MWG54_11800)	2278445..2279047	+	603	WP_000517260.1	hypothetical protein	-
MWG54_RS11805 (MWG54_11805)	2279289..2279540	-	252	WP_000827560.1	LuxR C-terminal-related transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T241900 WP_000624977.1 NZ_CP095377:2274049-2274786 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T241900 NZ_OU659164:19678-19959 [Providencia alcalifaciens]
ATGACTTATAAACTTGAATTTGATAGACGGGCGTTAAAAGAATGGCAAAAACTAGCCCCTCCGATTAGAAATCAATTTAA
AAAGAAACTGTTAGAACGTATTGAAAATCCTTGTGTTCCATCAGCTAAACTTAGTGGTAAAAATAATCGTTATAAAATAA
AACTACGATCATTAGGTTACCGCTTAATTTATGAAGTGATAGATGAAAAAATTATTCTGTTAGTCATTGCCGTAGGTAAA
AGAGAGGGCAATACTGTCTATTTTTCATCGAACGAACGCTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT241900 WP_000237818.1 NZ_CP095377:2274899-2275027 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT241900 NZ_OU659164:19437-19688 [Providencia alcalifaciens]
ATGACTTATCAAATTTTAAGTGATACTGCTGCGAGTATTACTGACTTGAAGAAAAATCCAATGGGCACTATTGCAGGTGG
TGAAGGGAACCCCGTAGCTATTCTTAATAGAAATGAACCTGCATTTTATTGTGTTCCCCCTGAACTATTTGCTTGGTTCA
TGGAGTTAGCTGAAGATGCTGAACTTAACAAAATTGCAGATGAACGATTAGAATCACTTGATGTAGTGAGTGTGAATATC
GATGACTTATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: W8YZL5