Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 240780..241422 Replicon chromosome
Accession NZ_CP095377
Organism Bacillus cereus strain SEM-15

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag MWG54_RS01365 Protein ID WP_000635965.1
Coordinates 241072..241422 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag MWG54_RS01360 Protein ID WP_000004570.1
Coordinates 240780..241067 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWG54_RS01335 (MWG54_01335) 236097..237059 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
MWG54_RS01340 (MWG54_01340) 237052..237624 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
MWG54_RS01345 (MWG54_01345) 237718..238077 + 360 WP_000583417.1 holo-ACP synthase -
MWG54_RS01350 (MWG54_01350) 238234..239184 + 951 WP_002060618.1 outer membrane lipoprotein carrier protein LolA -
MWG54_RS01355 (MWG54_01355) 239302..240471 + 1170 WP_000390617.1 alanine racemase -
MWG54_RS01360 (MWG54_01360) 240780..241067 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
MWG54_RS01365 (MWG54_01365) 241072..241422 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
MWG54_RS01370 (MWG54_01370) 241490..243658 + 2169 WP_246961769.1 Tex family protein -
MWG54_RS01375 (MWG54_01375) 243716..243832 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
MWG54_RS01380 (MWG54_01380) 244028..244486 + 459 WP_000344235.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T241899 WP_000635965.1 NZ_CP095377:241072-241422 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T241899 NZ_OU659163:c77192-76911 [Providencia alcalifaciens]
ATGACTTATAAACTTGAATTTGATAGACGGGCGTTAAAAGAATGGCAAAAACTAGCCCCTCCGATTAGAAATCAATTTAA
AAAGAAACTGTTAGAACGTATTGAAAATCCTTGTGTTCCATCAGCTAAACTTAGTGGTAAAAATAATCGTTATAAAATAA
AACTACGATCATTAGGTTACCGCTTAATTTATGAAGTGATAGATGAAAAAATTATTCTGTTAGTCATTGCCGTTGGTAAA
AGAGAGGGTAATACTGTCTATTTTTCATCGAACGAACGCTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT241899 WP_000004570.1 NZ_CP095377:240780-241067 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT241899 NZ_OU659163:c77433-77182 [Providencia alcalifaciens]
ATGACTTATCAAATTTTGAGTGATACTGCTGCGAGCATAACTGACTTGAAGAAAAATCCAATGGGCACTATTGCAGGTGG
TGAAGGGAACCCCGTAGCTATTCTTAATAGAAATGAACCTGCATTTTATTGTGTTCCCCCTGAACTATTTGCTTGGTTCA
TGGAGTTAGCTGAAGATGCTGAACTTAACAAAATTGCAGATGAACGATTAGAATCACTTGATGTAGTGAGTGTGAATATC
GATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References