Detailed information of TA system TA241895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 35367..36720
• Replicon: plasmid unnamed1
• Accession: NZ_CP095282
• Organism: Aeromonas hydrophila strain Ah2111

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: MUW98_RS23945
• Protein ID: WP_247141462.1
• Coordinates: 35367..36332 (-)
• Length: 322 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: -
• Locus tag: MUW98_RS23950
• Protein ID: WP_247146330.1
• Coordinates: 36322..36720 (-)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUW98_RS23925 (MUW98_23925)	33431..33613	-	183	WP_199416995.1	hypothetical protein	-
MUW98_RS23930 (MUW98_23930)	33778..33930	-	153	WP_005322083.1	Hok/Gef family protein	-
MUW98_RS23935 (MUW98_23935)	34440..34940	-	501	WP_199416981.1	hypothetical protein	-
MUW98_RS23940 (MUW98_23940)	34956..35321	-	366	WP_042468255.1	hypothetical protein	-
MUW98_RS23945 (MUW98_23945)	35367..36332	-	966	WP_247141462.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
MUW98_RS23950 (MUW98_23950)	36322..36720	-	399	WP_247146330.1	DUF6088 family protein	Antitoxin
MUW98_RS23955 (MUW98_23955)	37411..38358	+	948	WP_031285326.1	IS30 family transposase	-
MUW98_RS23960 (MUW98_23960)	38737..39183	+	447	WP_247141458.1	DNA repair protein RadC	-
MUW98_RS23965 (MUW98_23965)	39223..39585	+	363	WP_011898725.1	hypothetical protein	-
MUW98_RS23970 (MUW98_23970)	39907..40227	-	321	WP_129712433.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..78294	78294
	-	flank	IS/Tn	-	-	37411..38358	947

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (38-302)

Antitoxin

• Protein of unknown function DUF6088 (7-128)

Sequences

Toxin

Protein

• Length: 322 a.a.
• Molecular weight: 36384.42 Da
• Isoelectric Point: 5.1489

>T241895 WP_247141462.1 NZ_CP095282:c36332-35367 [Aeromonas hydrophila]
MQPKDSSIFADVSDYFGIDNPAIAEKDYYVVQLLKLLASHSCQHHTMVFAGGTALAKADVKLQRMSEDVDIKLSVNDSAK
DESRSAMKRHRKAIRDGLIEELNATGVFQVEKAEVTCRDEHRYIEMPVRYPQAFSKAPCLRPFIKLELIETDLLAGHNPM
PICSLHNEAMQQGPEVPAFNTVPLISTQAEKVLSMLRRTASVKHDPERLDDESLVRHIYDTYRLQQSTTFDLAELAGLIA
EGMKMDRDRYGPQHPEFAEDPIGVMRFGLDVIAGDSLYKDRYEAFVKPMVYGDALTWDEAFRVFEAVARQALDHIEQHEL
I

Nucleotide

• Length: 966 bp

>T241895 NZ_OU659151:c137091-136810 [Providencia alcalifaciens]
ATGACTTATAAACTTGAATTTGATAGACGGGCGTTAAAAGAATGGCAAAAACTAGCCCCTCCGATTAGAAATCAATTTAA
AAAGAAACTGTTAGAACGTCTTGAAAATCCTTGTGTTCCATCAGCTAAACTTAGTGGTAAAAATAATCGTTATAAAATAA
AACTACGATCATTAGGTTACCGCTTAATTTATGAAGTGATAGATGAAAAAATTATTCTGTTAGTCATTGCCGTAGGTAAA
AGAGAGGGCAATACTGTCTATTTTTCATCGAACGAACGCTAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14774.32 Da
• Isoelectric Point: 10.9709

>AT241895 WP_247146330.1 NZ_CP095282:c36720-36322 [Aeromonas hydrophila]
MTIADRVEARIKRSKRDVFLREDFDDLGGYDQIGRALRQMCEKGLLLKVGYGVYAKARRNRINSRLMIASPGGPDAVLIA
TLDRLKVPYELNGLTAAYAAGKSTQVPAFMEVKPKKRFSRKLKIGNRVFNAA

Nucleotide

• Length: 399 bp

>AT241895 NZ_OU659151:c137332-137081 [Providencia alcalifaciens]
ATGACTTATCAAATTTTAAGTGATACTGCTGCGAGTATTACTGACTTGAAGAAAAATCCAATGGGCACTATTGCAGGTGG
TGAAGGGAACCCCGTAGCTATTCTTAATAGAAATGAACCTGCATTTTATTGTGTTCCCCCTGAACTATTTGCTTGGTTCA
TGGAGTTAGCTGAAGATGCTGAACTTAACAAAATTGCAGATGAACGATTAGAATCACTTGATGTAGTGAGTGTGAACATC
GATGACTTATAA