Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/FR47-DUF1778
Location 35103..35900 Replicon plasmid pHD8642-1
Accession NZ_CP095277
Organism Enterobacter hormaechei subsp. xiangfangensis strain HD8642

Toxin (Protein)


Gene name KacT Uniprot ID F5S3R9
Locus tag MU851_RS23430 Protein ID WP_006812498.1
Coordinates 35376..35900 (+) Length 175 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID F5S3R8
Locus tag MU851_RS23425 Protein ID WP_006812497.1
Coordinates 35103..35372 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU851_RS23400 (MU851_23365) 30477..31817 - 1341 WP_006812582.1 DnaB-like helicase C-terminal domain-containing protein -
MU851_RS23405 (MU851_23370) 31878..32603 - 726 WP_252035768.1 hypothetical protein -
MU851_RS23410 (MU851_23375) 32867..33664 + 798 WP_077929499.1 DUF2971 domain-containing protein -
MU851_RS23415 (MU851_23380) 33706..34059 - 354 WP_160859104.1 hypothetical protein -
MU851_RS23420 (MU851_23385) 34065..34730 - 666 WP_023315962.1 AAA family ATPase -
MU851_RS23425 (MU851_23390) 35103..35372 + 270 WP_006812497.1 DUF1778 domain-containing protein Antitoxin
MU851_RS23430 (MU851_23395) 35376..35900 + 525 WP_006812498.1 GNAT family N-acetyltransferase Toxin
MU851_RS23435 (MU851_23400) 35927..36091 - 165 WP_006812499.1 hypothetical protein -
MU851_RS23440 (MU851_23405) 36084..36335 - 252 WP_000147960.1 hypothetical protein -
MU851_RS23445 (MU851_23410) 36337..37029 - 693 WP_006812500.1 membrane protein -
MU851_RS23450 (MU851_23415) 37043..37366 - 324 WP_000064173.1 hypothetical protein -
MU851_RS23455 (MU851_23420) 37441..38229 - 789 WP_046340563.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..107477 107477


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-149)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 175 a.a.        Molecular weight: 19356.13 Da        Isoelectric Point: 8.9844

>T241890 WP_006812498.1 NZ_CP095277:35376-35900 [Enterobacter hormaechei subsp. xiangfangensis]
VANLTIEMFSEEAVYDFSCFDCGETSLNDFLNNRLAQQHSGRILRGYLLLTRDPIPKVMGFYTLSGSCFARNTLPSNTQQ
RKVPYSDAPSVTLGRLAIDKSIQRQGYGETLVAHAMKVVYRASLAVGIYALFVDAKNENAMRFYKQLGFTPLTGDNANSL
FYPTKGIEKLFGGK

Download         Length: 525 bp

>T241890 NZ_OU659133:c66254-65976 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATGCCACACGAAGTCCGTCGGATTGTGGTGGTGAATTATGAGATACGTTATGAACTCATTGATTCGACAATC
TATATTTTACGGATATGGCATACACGAGAAGATCGGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10114.51 Da        Isoelectric Point: 4.8483

>AT241890 WP_006812497.1 NZ_CP095277:35103-35372 [Enterobacter hormaechei subsp. xiangfangensis]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNIVMDAISNPPAPNDRLKR
AAERLQSME

Download         Length: 270 bp

>AT241890 NZ_OU659133:c66535-66245 [Providencia alcalifaciens]
ATGAAAAACAGCTCGGTACAAAATAAGACAAAAGTGCTAACAGCACATGTGCCTTTAACGATGGCAGATAAGATTGATGA
AATAGCCGAACGTCTTGAACGTTCCCGTGGATGGGTCATGAAGCAAGCGTTGTCTACGTGGATAGCTCAAGAGGAGATGC
GGGACACATTGACACGAGAAGCGTTATTGGATGTTGAATCTGGCCAAGTGATAGATAATCAGTCTGTGCAAGATTGGGCG
GCTAGCTTGAATACTGAACTACCATTACCGGTGCCACGCTGATGGAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0QWY8

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0QUA3

References