Detailed information of TA system TA241889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4758758..4759374
• Replicon: chromosome
• Accession: NZ_CP095276
• Organism: Enterobacter hormaechei subsp. xiangfangensis strain HD8642

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MU851_RS22800
• Protein ID: WP_077266491.1
• Coordinates: 4758758..4759129 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: MU851_RS22805
• Protein ID: WP_015569912.1
• Coordinates: 4759132..4759374 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU851_RS22785 (MU851_22755)	4756258..4757160	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
MU851_RS22790 (MU851_22760)	4757157..4757792	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
MU851_RS22795 (MU851_22765)	4757789..4758718	+	930	WP_003861956.1	formate dehydrogenase accessory protein FdhE	-
MU851_RS22800 (MU851_22770)	4758758..4759129	-	372	WP_077266491.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU851_RS22805 (MU851_22775)	4759132..4759374	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
MU851_RS22810 (MU851_22780)	4759573..4760493	+	921	WP_047062215.1	alpha/beta hydrolase	-
MU851_RS22815 (MU851_22785)	4760502..4761443	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
MU851_RS22820 (MU851_22790)	4761488..4761925	-	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
MU851_RS22825 (MU851_22795)	4761922..4762803	-	882	WP_003861949.1	virulence factor BrkB family protein	-
MU851_RS22830 (MU851_22800)	4762797..4763396	-	600	WP_003861947.1	glucose-1-phosphatase	-
MU851_RS22835 (MU851_22805)	4763515..4764315	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13723.85 Da
• Isoelectric Point: 6.4882

>T241889 WP_077266491.1 NZ_CP095276:c4759129-4758758 [Enterobacter hormaechei subsp. xiangfangensis]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T241889 NZ_OU659133:c66254-65976 [Providencia alcalifaciens]
ATGGAATTAAAATGGACGAGTAAGGCATTATCGGATCTTTCTCGTGTATATGATTTTTTAGCGTTATCAAACTCAGTCGC
TGCCGCGCGTGTTGTGCAAACATTGACGAAAGCCCCCATGTTGTTGTTGATAAAAAACCCCCGAATGGGAGAGCAATTGT
TTCAATTTATGCCACACGAAGTCCGTCGGATTGTGGTGGTGAATTATGAGATACGTTATGAACTCATTGATTCGACAATC
TATATTTTACGGATATGGCATACACGAGAAGATCGGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT241889 WP_015569912.1 NZ_CP095276:c4759374-4759132 [Enterobacter hormaechei subsp. xiangfangensis]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT241889 NZ_OU659133:c65976-65833 [Providencia alcalifaciens]
ATGAGACAATTTAAAGTATCTCACCTTGGTGAAATTATCGCTAGAGACTTGGCTGATATGGGGATTAGTGGTCGACGTTT
CGCGCTCAATATTGATGTCACTCCAGCGACCGCTGGCAATTCGAATTGCTGCTGCATTAGATAG

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3