Detailed information of TA system TA241866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 89189..89814
• Replicon: plasmid pEC9682-1
• Accession: NZ_CP095272
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MU524_RS25785
• Protein ID: WP_000911313.1
• Coordinates: 89189..89587 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: V0VCB2
• Locus tag: MU524_RS25790
• Protein ID: WP_000450520.1
• Coordinates: 89587..89814 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS25770 (85514)	85514..86011	+	498	WP_000605857.1	entry exclusion protein	-
MU524_RS25775 (86043)	86043..86774	+	732	WP_023565183.1	conjugal transfer complement resistance protein TraT	-
MU524_RS25780 (87027)	87027..89180	+	2154	WP_000009324.1	type IV conjugative transfer system coupling protein TraD	-
MU524_RS25785 (89189)	89189..89587	-	399	WP_000911313.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU524_RS25790 (89587)	89587..89814	-	228	WP_000450520.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / aph(3'')-Ib / sul2 / tet(B) / catA1 / dfrA17	iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..146265	146265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (8-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14901.15 Da
• Isoelectric Point: 7.8605

>T241866 WP_000911313.1 NZ_CP095272:c89587-89189 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHS
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T241866 NZ_OU659118:3897636-3897890 [Providencia alcalifaciens]
ATGAAAACCATTTCCTTTAGTGATGCTAGAAGCAATTTGAAAGCAGTGCTTGATAGAGTTGTTGATGATATGGATACGAC
GATTATAACCCGCCGTGATTCTGAAGCGGCTGTAGTTATGTCCTTGGAATACTACAATAGTTTAATGGAAACCATCTATC
TACTGCGCTCTCCTGCAAATGCAGAGCATCTCAACAAATCCATCGAGCAATTTAAAGCGGGTAAGATTTCAGAGAGAAAG
CTAATCAATGAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8596.63 Da
• Isoelectric Point: 4.7513

>AT241866 WP_000450520.1 NZ_CP095272:c89814-89587 [Escherichia coli]
METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGHNVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT241866 NZ_OU659118:3897883-3898143 [Providencia alcalifaciens]
ATGAGTAGACTTCTAACATGGACGGATCAAGCTTGGGAAGACTATCTATACTGGCAAAACCAAGATAAAAAGACATTAAA
GCGGATAAATAAACTCATTGAAGACACGAAAAGAACGCCATTCAGCGGTATAGGTAAACCAGAGCCACTAAAAGAAAACT
TGGCTGGCTTCTGGTCTCGTAGAATAGATGAAACAAACAGGCTAGTTTATGCAATCGAAAATACCGCATTAGTTATCATT
GCCTGCCGCTATCACTACTGA

Structures

Antitoxin

• AlphaFold DB: V0VCB2