Detailed information of TA system TA241860

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4376139..4376974
• Replicon: chromosome
• Accession: NZ_CP095271
• Organism: Escherichia coli strain EC9682

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: Q1R2L4
• Locus tag: MU524_RS21615
• Protein ID: WP_001094426.1
• Coordinates: 4376139..4376516 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: Q1R2L5
• Locus tag: MU524_RS21620
• Protein ID: WP_001285575.1
• Coordinates: 4376606..4376974 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU524_RS21590 (4371642)	4371642..4371821	+	180	Protein_4230	peptidase	-
MU524_RS21595 (4372220)	4372220..4373983	-	1764	WP_242794220.1	hypothetical protein	-
MU524_RS21600 (4375211)	4375211..4375360	-	150	Protein_4232	hypothetical protein	-
MU524_RS21605 (4375445)	4375445..4375642	-	198	WP_000839272.1	DUF957 domain-containing protein	-
MU524_RS21610 (4375654)	4375654..4376142	-	489	WP_000761683.1	DUF5983 family protein	-
MU524_RS21615 (4376139)	4376139..4376516	-	378	WP_001094426.1	TA system toxin CbtA family protein	Toxin
MU524_RS21620 (4376606)	4376606..4376974	-	369	WP_001285575.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MU524_RS21625 (4377054)	4377054..4377275	-	222	WP_000692350.1	DUF987 domain-containing protein	-
MU524_RS21630 (4377362)	4377362..4377838	-	477	WP_001298074.1	RadC family protein	-
MU524_RS21635 (4377853)	4377853..4378338	-	486	WP_097494582.1	antirestriction protein	-
MU524_RS21640 (4378429)	4378429..4379247	-	819	WP_001175160.1	DUF932 domain-containing protein	-
MU524_RS21645 (4379337)	4379337..4379570	-	234	WP_001278290.1	DUF905 family protein	-
MU524_RS21650 (4379576)	4379576..4380253	-	678	WP_001097301.1	hypothetical protein	-
MU524_RS21655 (4380401)	4380401..4381081	-	681	WP_001282919.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	4365558..4390848	25290

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14072.99 Da
• Isoelectric Point: 7.3523

>T241860 WP_001094426.1 NZ_CP095271:c4376516-4376139 [Escherichia coli]
MNTLPDTHIREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T241860 NZ_OU659114:146164-146442 [Providencia alcalifaciens]
ATGATAAAATCGTTTAAACATAAAGGGTTGAAACAACTCTTTGAAAAAGGAATAACTTCAGGGGTTCCGGCTCAAGATGC
TGAACGGATCAACGACCGTTTACAAGCAATAGATATCGCTAACGATATTCGTGAATTAGACCGCCAAATTTACAAATTGC
ATCCACTGAAAGGTGACAGAGAAGGTTATTGGTCAATAACAGTGCGAGCGAATTGGCGCATTACTTTTCAATTCATCAAT
GGTGATGCTTACATCTTAAATTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13709.52 Da
• Isoelectric Point: 6.6258

>AT241860 WP_001285575.1 NZ_CP095271:c4376974-4376606 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT241860 NZ_OU659114:146442-146756 [Providencia alcalifaciens]
ATGAGACAGTTTAAAATATCTCACCCAGGTGAAATTATTGCACGAGATTTGGCTGATATGGGGATCAGTGGTCGACGTTT
TGCATTAAATATTGGTGTAACGCCAGCAACAGTTTCCCGTTTTTTATCAGGTAAAACAGCATTGACTCCCGCATTGGCAA
TCCGTATCGCCGCTGCACTAGGAAGTACTCCTGAATTTTGGTTACGTTTACAAAGTGCTTATGATTTACGTCAATTAGAA
AATCAAATTGATACGTCCGATATTATTTTGTATGAAGAGGCGCCTGATAGCGCTAAAGGCATATCCAAACATTAG

Structures

Toxin

• AlphaFold DB: A0A454ABD6

Antitoxin

• AlphaFold DB: A0A454ABF5