Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3768142..3768760 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MU524_RS18775 Protein ID WP_001291435.1
Coordinates 3768542..3768760 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MU524_RS18770 Protein ID WP_000344800.1
Coordinates 3768142..3768516 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS18760 (3763232) 3763232..3764425 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MU524_RS18765 (3764448) 3764448..3767597 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
MU524_RS18770 (3768142) 3768142..3768516 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MU524_RS18775 (3768542) 3768542..3768760 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MU524_RS18780 (3768934) 3768934..3769485 + 552 WP_000102543.1 maltose O-acetyltransferase -
MU524_RS18785 (3769601) 3769601..3770071 + 471 WP_000136192.1 YlaC family protein -
MU524_RS18790 (3770235) 3770235..3771785 + 1551 WP_001350616.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MU524_RS18795 (3771827) 3771827..3772180 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MU524_RS18805 (3772559) 3772559..3772870 + 312 WP_000409908.1 MGMT family protein -
MU524_RS18810 (3772901) 3772901..3773473 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241856 WP_001291435.1 NZ_CP095271:3768542-3768760 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T241856 NZ_OU594967:1545854-1546108 [Celerinatantimonas diazotrophica]
ATGATGTTAGTGTGGGCATCCAGTGCCTGGGAAGATTATTTATATTGGCAGCAAACCGATAAAAAGATATTAAAACGTAT
CAATCAGCTCATTAAAGAAATTAGTCGAGAACCATTTGAAGGCATTGGTGATCCTGAGCCATTAAAACATCAATGGACAG
GCTATTGGTCTCGACGGATTAATAGAGAACATCGGCTTGTTTATAAAGCTAAAGAAAACGAAGTTTTGATTGCTCAATGT
CGGTATCACTACTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT241856 WP_000344800.1 NZ_CP095271:3768142-3768516 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT241856 NZ_OU594967:1545603-1545857 [Celerinatantimonas diazotrophica]
ATGGATGTAATGAGCTATTCACAATTTCGAGCGCAGTTAGCTAAAACGCTTGATGATGTGAACGATGACCACAAGCCGGT
GATGATCACTCGACAAAATGGTAAGCCTGCGATTGTCATGAGCGTTGAAGATTTCAAAGCGTATGAAGAGACTGCCTATC
TTATGGCGAGCCCTAAGAACGCACAGCGGCTGAACGCGGCCATTTCAGAACTAGAAGGTGGTCATGGCAGTGAAAAGGGG
CTGATTGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References