Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1933456..1934287 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag MU524_RS09435 Protein ID WP_000854814.1
Coordinates 1933456..1933830 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag MU524_RS09440 Protein ID WP_001285586.1
Coordinates 1933919..1934287 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS09395 (1928854) 1928854..1930019 + 1166 Protein_1846 serine-type D-Ala-D-Ala carboxypeptidase DacD -
MU524_RS09400 (1930137) 1930137..1930610 + 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
MU524_RS09405 (1930808) 1930808..1931866 + 1059 WP_001200890.1 FUSC family protein -
MU524_RS09410 (1932038) 1932038..1932367 + 330 WP_000450409.1 DUF496 family protein -
MU524_RS09415 (1932468) 1932468..1932791 - 324 WP_223216378.1 EutP/PduV family microcompartment system protein -
MU524_RS09420 (1932770) 1932770..1932850 + 81 WP_023441679.1 hypothetical protein -
MU524_RS09425 (1933139) 1933139..1933219 - 81 Protein_1852 hypothetical protein -
MU524_RS09430 (1933265) 1933265..1933459 - 195 WP_000988601.1 DUF5983 family protein -
MU524_RS09435 (1933456) 1933456..1933830 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
MU524_RS09440 (1933919) 1933919..1934287 - 369 WP_001285586.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
MU524_RS09445 (1934361) 1934361..1934582 - 222 WP_000692323.1 DUF987 domain-containing protein -
MU524_RS09450 (1934651) 1934651..1935127 - 477 WP_001351157.1 RadC family protein -
MU524_RS09455 (1935143) 1935143..1935616 - 474 WP_000855074.1 antirestriction protein -
MU524_RS09460 (1935879) 1935879..1936700 - 822 WP_001234570.1 DUF932 domain-containing protein -
MU524_RS09465 (1936921) 1936921..1937277 - 357 Protein_1860 hypothetical protein -
MU524_RS09470 (1937363) 1937363..1938109 - 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T241836 WP_000854814.1 NZ_CP095271:c1933830-1933456 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T241836 NZ_OU342919:4451317-4451424 [Escherichia coli]
ATGACGCTCGCGCAGTTTACCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT241836 WP_001285586.1 NZ_CP095271:c1934287-1933919 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT241836 NZ_OU342919:c4451270-4451204 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References