Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1040231..1040814 Replicon chromosome
Accession NZ_CP095271
Organism Escherichia coli strain EC9682

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag MU524_RS04965 Protein ID WP_000254750.1
Coordinates 1040479..1040814 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag MU524_RS04960 Protein ID WP_000581937.1
Coordinates 1040231..1040479 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU524_RS04950 (1036570) 1036570..1037871 + 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
MU524_RS04955 (1037919) 1037919..1040153 + 2235 WP_000226815.1 GTP pyrophosphokinase -
MU524_RS04960 (1040231) 1040231..1040479 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
MU524_RS04965 (1040479) 1040479..1040814 + 336 WP_000254750.1 endoribonuclease MazF Toxin
MU524_RS04970 (1040886) 1040886..1041677 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
MU524_RS04975 (1041905) 1041905..1043542 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
MU524_RS04980 (1043630) 1043630..1044928 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T241834 WP_000254750.1 NZ_CP095271:1040479-1040814 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T241834 NZ_OU342919:4450247-4450354 [Escherichia coli]
ATGACGCTCGCGCAGTTTACCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT241834 WP_000581937.1 NZ_CP095271:1040231-1040479 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT241834 NZ_OU342919:c4450200-4450134 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References