Detailed information of TA system TA241831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 10924..11504
• Replicon: plasmid p21091025_3
• Accession: NZ_CP095267
• Organism: Klebsiella pneumoniae strain 21091025

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: MU321_RS28430
• Protein ID: WP_071177730.1
• Coordinates: 10924..11238 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: MU321_RS28435
• Protein ID: WP_000093040.1
• Coordinates: 11226..11504 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU321_RS28385 (MU321_28385)	6871..7050	+	180	WP_247191849.1	hypothetical protein	-
MU321_RS28390 (MU321_28390)	7032..7841	+	810	WP_247191850.1	type IV secretory system conjugative DNA transfer family protein	-
MU321_RS28395 (MU321_28395)	7763..8413	+	651	WP_247191851.1	hypothetical protein	-
MU321_RS28400 (MU321_28400)	8418..8621	+	204	WP_247191853.1	TraM recognition domain-containing protein	-
MU321_RS28405 (MU321_28405)	8561..8833	+	273	WP_228742589.1	hypothetical protein	-
MU321_RS28410 (MU321_28410)	8833..9564	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
MU321_RS28415 (MU321_28415)	9571..10101	+	531	WP_071177729.1	hypothetical protein	-
MU321_RS28420 (MU321_28420)	10031..10306	-	276	WP_247191852.1	Rop family plasmid primer RNA-binding protein	-
MU321_RS28425 (MU321_28425)	10332..10760	-	429	WP_001140599.1	hypothetical protein	-
MU321_RS28430 (MU321_28430)	10924..11238	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MU321_RS28435 (MU321_28435)	11226..11504	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
MU321_RS28440 (MU321_28440)	11679..12044	-	366	WP_072354022.1	TonB family protein	-
MU321_RS28445 (MU321_28445)	12041..12412	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
MU321_RS28450 (MU321_28450)	12685..12930	-	246	WP_032440458.1	hypothetical protein	-
MU321_RS28455 (MU321_28455)	13575..15058	+	1484	Protein_21	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..23759	23759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T241831 WP_071177730.1 NZ_CP095267:c11238-10924 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T241831 NZ_OU342919:3789026-3789129 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT241831 WP_000093040.1 NZ_CP095267:c11504-11226 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT241831 NZ_OU342919:c3789167-3788896 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1