Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4195478..4196097 Replicon chromosome
Accession NZ_CP095264
Organism Klebsiella pneumoniae strain 21091025

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MU321_RS20900 Protein ID WP_002892050.1
Coordinates 4195879..4196097 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MU321_RS20895 Protein ID WP_002892066.1
Coordinates 4195478..4195852 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU321_RS20885 (MU321_20885) 4190630..4191823 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MU321_RS20890 (MU321_20890) 4191846..4194992 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MU321_RS20895 (MU321_20895) 4195478..4195852 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MU321_RS20900 (MU321_20900) 4195879..4196097 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MU321_RS20905 (MU321_20905) 4196256..4196822 + 567 WP_002892030.1 maltose O-acetyltransferase -
MU321_RS20910 (MU321_20910) 4196794..4196934 - 141 WP_004147370.1 hypothetical protein -
MU321_RS20915 (MU321_20915) 4196955..4197425 + 471 WP_002892026.1 YlaC family protein -
MU321_RS20920 (MU321_20920) 4197400..4198851 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MU321_RS20925 (MU321_20925) 4198952..4199650 + 699 WP_002892021.1 GNAT family protein -
MU321_RS20930 (MU321_20930) 4199647..4199787 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MU321_RS20935 (MU321_20935) 4199787..4200050 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241822 WP_002892050.1 NZ_CP095264:4195879-4196097 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T241822 NZ_OU342919:c1496603-1496292 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT241822 WP_002892066.1 NZ_CP095264:4195478-4195852 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT241822 NZ_OU342919:c1496291-1496001 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References