Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 87827..88737 Replicon plasmid p21080937_2
Accession NZ_CP095255
Organism Klebsiella pneumoniae strain 21080937

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag MU320_RS27770 Protein ID WP_004026354.1
Coordinates 88267..88737 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag MU320_RS27765 Protein ID WP_004026357.1
Coordinates 87827..88270 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU320_RS27735 (MU320_27735) 82855..83376 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
MU320_RS27740 (MU320_27740) 83373..84326 + 954 Protein_98 fec operon regulator FecR -
MU320_RS27745 (MU320_27745) 84413..86529 + 2117 Protein_99 TonB-dependent Fe(3+) dicitrate receptor FecA -
MU320_RS27750 (MU320_27750) 86604..86729 + 126 Protein_100 Fe3+-citrate ABC transporter substrate-binding protein -
MU320_RS27755 (MU320_27755) 86904..87125 + 222 WP_004213643.1 DUF2188 domain-containing protein -
MU320_RS27760 (MU320_27760) 87223..87726 + 504 Protein_102 DUF4113 domain-containing protein -
MU320_RS27765 (MU320_27765) 87827..88270 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
MU320_RS27770 (MU320_27770) 88267..88737 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
MU320_RS27775 (MU320_27775) 88886..89583 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
MU320_RS27780 (MU320_27780) 90969..91619 - 651 WP_068893702.1 DUF1173 family protein -
MU320_RS27785 (MU320_27785) 91652..91918 - 267 WP_223175074.1 DUF1173 family protein -
MU320_RS27790 (MU320_27790) 92096..92590 + 495 WP_004212794.1 thermonuclease family protein -
MU320_RS27795 (MU320_27795) 92927..93325 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aadA5 / qacE / sul1 rmpA / rmpA / iroN / iroD / iroC / iroB 1..132724 132724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T241801 WP_004026354.1 NZ_CP095255:88267-88737 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T241801 NZ_OU016039:c22483-21620 [Enterococcus faecium]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT241801 WP_004026357.1 NZ_CP095255:87827-88270 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT241801 NZ_OU016039:c22757-22485 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References