Detailed information of TA system TA241799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 81606..82276
• Replicon: plasmid p21080937_1
• Accession: NZ_CP095254
• Organism: Klebsiella pneumoniae strain 21080937

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MU320_RS26850
• Protein ID: WP_004213072.1
• Coordinates: 81606..82049 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MU320_RS26855
• Protein ID: WP_004213073.1
• Coordinates: 82046..82276 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU320_RS26815 (MU320_26815)	77017..77292	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
MU320_RS26820 (MU320_26820)	77355..77846	+	492	WP_011251327.1	DM13 domain-containing protein	-
MU320_RS26825 (MU320_26825)	77895..78815	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
MU320_RS26830 (MU320_26830)	78906..79309	+	404	Protein_80	GAF domain-containing protein	-
MU320_RS26835 (MU320_26835)	79827..80462	-	636	Protein_81	mucoid phenotype regulator RmpA2	-
MU320_RS26840 (MU320_26840)	80879..81183	+	305	Protein_82	transposase	-
MU320_RS26845 (MU320_26845)	81206..81457	-	252	WP_186987481.1	hypothetical protein	-
MU320_RS26850 (MU320_26850)	81606..82049	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU320_RS26855 (MU320_26855)	82046..82276	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MU320_RS26860 (MU320_26860)	82884..84017	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MU320_RS26865 (MU320_26865)	84033..84326	+	294	WP_004213076.1	hypothetical protein	-
MU320_RS26870 (MU320_26870)	84316..84522	-	207	WP_004213077.1	hypothetical protein	-
MU320_RS26875 (MU320_26875)	84874..85164	+	291	WP_004213078.1	hypothetical protein	-
MU320_RS26880 (MU320_26880)	85154..86053	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2	1..157481	157481

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T241799 WP_004213072.1 NZ_CP095254:c82049-81606 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T241799 NZ_OU016037:48924-49274 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTAAAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT241799 WP_004213073.1 NZ_CP095254:c82276-82046 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT241799 NZ_OU016037:48667-48930 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620