Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2280701..2281326 Replicon chromosome
Accession NZ_CP095253
Organism Klebsiella pneumoniae strain 21080937

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU320_RS11060 Protein ID WP_002882817.1
Coordinates 2280701..2281084 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU320_RS11065 Protein ID WP_004150355.1
Coordinates 2281084..2281326 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU320_RS11045 (MU320_11045) 2278067..2278969 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU320_RS11050 (MU320_11050) 2278966..2279601 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU320_RS11055 (MU320_11055) 2279598..2280527 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU320_RS11060 (MU320_11060) 2280701..2281084 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU320_RS11065 (MU320_11065) 2281084..2281326 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU320_RS11070 (MU320_11070) 2281531..2282448 + 918 WP_002882812.1 alpha/beta hydrolase -
MU320_RS11075 (MU320_11075) 2282462..2283403 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU320_RS11080 (MU320_11080) 2283448..2283885 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU320_RS11085 (MU320_11085) 2283882..2284742 - 861 WP_002882807.1 virulence factor BrkB family protein -
MU320_RS11090 (MU320_11090) 2284736..2285335 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241785 WP_002882817.1 NZ_CP095253:c2281084-2280701 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241785 NZ_OU015709:45704-46054 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241785 WP_004150355.1 NZ_CP095253:c2281326-2281084 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241785 NZ_OU015709:45447-45710 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References