Detailed information of TA system TA241779

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 177549..178219
• Replicon: plasmid p21080534_1
• Accession: NZ_CP095248
• Organism: Klebsiella pneumoniae strain 21080534

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: MU311_RS27985
• Protein ID: WP_004213072.1
• Coordinates: 177776..178219 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: MU311_RS27980
• Protein ID: WP_004213073.1
• Coordinates: 177549..177779 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU311_RS27945 (172718)	172718..173239	-	522	WP_013214008.1	hypothetical protein	-
MU311_RS27950 (173297)	173297..173689	-	393	WP_011977811.1	hypothetical protein	-
MU311_RS27955 (173726)	173726..174670	-	945	WP_011977810.1	hypothetical protein	-
MU311_RS27960 (174831)	174831..175094	-	264	Protein_224	hypothetical protein	-
MU311_RS27965 (175303)	175303..175509	+	207	WP_004213077.1	hypothetical protein	-
MU311_RS27970 (175499)	175499..175792	-	294	WP_004213076.1	hypothetical protein	-
MU311_RS27975 (175808)	175808..176941	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
MU311_RS27980 (177549)	177549..177779	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MU311_RS27985 (177776)	177776..178219	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
MU311_RS27990 (178368)	178368..178619	+	252	WP_186987481.1	hypothetical protein	-
MU311_RS27995 (178642)	178642..178946	-	305	Protein_231	transposase	-
MU311_RS28000 (179363)	179363..179998	+	636	Protein_232	mucoid phenotype regulator RmpA2	-
MU311_RS28005 (180516)	180516..180919	-	404	Protein_233	GAF domain-containing protein	-
MU311_RS28010 (181010)	181010..181930	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
MU311_RS28015 (181979)	181979..182470	-	492	WP_011251327.1	DM13 domain-containing protein	-
MU311_RS28020 (182533)	182533..182808	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaSHV-12	rmpA2 / iutA / iucD / iucC / iucB / iucA / iucA	1..210914	210914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T241779 WP_004213072.1 NZ_CP095248:177776-178219 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T241779 NZ_OU015352:50395-50652 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT241779 WP_004213073.1 NZ_CP095248:177549-177779 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT241779 NZ_OU015352:50133-50402 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620