Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5345049..5345674 Replicon chromosome
Accession NZ_CP095247
Organism Klebsiella pneumoniae strain 21080534

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU311_RS26475 Protein ID WP_002882817.1
Coordinates 5345049..5345432 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU311_RS26480 Protein ID WP_004150355.1
Coordinates 5345432..5345674 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU311_RS26460 (MU311_26460) 5342415..5343317 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU311_RS26465 (MU311_26465) 5343314..5343949 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU311_RS26470 (MU311_26470) 5343946..5344875 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU311_RS26475 (MU311_26475) 5345049..5345432 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU311_RS26480 (MU311_26480) 5345432..5345674 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU311_RS26485 (MU311_26485) 5345879..5346796 + 918 WP_002882812.1 alpha/beta hydrolase -
MU311_RS26490 (MU311_26490) 5346810..5347751 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU311_RS26495 (MU311_26495) 5347796..5348233 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU311_RS26500 (MU311_26500) 5348230..5349090 - 861 WP_002882807.1 virulence factor BrkB family protein -
MU311_RS26505 (MU311_26505) 5349084..5349683 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241769 WP_002882817.1 NZ_CP095247:c5345432-5345049 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241769 NZ_OU015342:c4801421-4801110 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241769 WP_004150355.1 NZ_CP095247:c5345674-5345432 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241769 NZ_OU015342:c4801109-4800819 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References