Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4147622..4148241 Replicon chromosome
Accession NZ_CP095247
Organism Klebsiella pneumoniae strain 21080534

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag MU311_RS20730 Protein ID WP_002892050.1
Coordinates 4148023..4148241 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag MU311_RS20725 Protein ID WP_002892066.1
Coordinates 4147622..4147996 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU311_RS20715 (MU311_20715) 4142774..4143967 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MU311_RS20720 (MU311_20720) 4143990..4147136 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
MU311_RS20725 (MU311_20725) 4147622..4147996 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
MU311_RS20730 (MU311_20730) 4148023..4148241 + 219 WP_002892050.1 HHA domain-containing protein Toxin
MU311_RS20735 (MU311_20735) 4148400..4148966 + 567 WP_002892030.1 maltose O-acetyltransferase -
MU311_RS20740 (MU311_20740) 4148938..4149078 - 141 WP_004147370.1 hypothetical protein -
MU311_RS20745 (MU311_20745) 4149099..4149569 + 471 WP_002892026.1 YlaC family protein -
MU311_RS20750 (MU311_20750) 4149544..4150995 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
MU311_RS20755 (MU311_20755) 4151096..4151794 + 699 WP_002892021.1 GNAT family protein -
MU311_RS20760 (MU311_20760) 4151791..4151931 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
MU311_RS20765 (MU311_20765) 4151931..4152194 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T241765 WP_002892050.1 NZ_CP095247:4148023-4148241 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T241765 NZ_OU015342:3724796-3725071 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAATGCCTCCGGCGTCAGCGTCCGCCATATCAACAGCGAAACCTGCATGACCGCCTGTTACTCACAAATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCACGGCGTCACCGTGAAGATCTCCGAGGGGTGTT
TGATCCTGATTGCAGAGACGGACGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATT
AAGGCGGGGGTAAATAATGTGGTGAACGGGGATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT241765 WP_002892066.1 NZ_CP095247:4147622-4147996 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT241765 NZ_OU015342:c3725061-3724639 [Salmonella enterica subsp. enterica serovar Typhimurium]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCGTCCGTCTCTGCAATCAGGATCAAACACCCCTCGGAGATCTTCACGGTGACGCCGTGCCCGGTCTCAAATCCCGCTT
CTTCCTGCCAGTCACCCTTAAGGTGCTGGCTGGGGATTTGTGAGTAACAGGCGGTCATGCAGGTTTCGCTGTTGATATGG
CGGACGCTGACGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTATTTATCCGCCGGATGCTGGCTGATTGTGGA
GCTCAGGGTGAGTGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCCCGGCCAGCCCCGGCCGGTGATGAGCA
GCCGGCTGCCGGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References