Detailed information of TA system TA241751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6524..7104
• Replicon: plasmid p21080237_6
• Accession: NZ_CP095246
• Organism: Klebsiella pneumoniae strain 21080237

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: MU300_RS29475
• Protein ID: WP_071177730.1
• Coordinates: 6790..7104 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: MU300_RS29470
• Protein ID: WP_000093040.1
• Coordinates: 6524..6802 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU300_RS29440 (MU300_29435)	2584..2733	-	150	WP_032440454.1	colicin release lysis protein	-
MU300_RS29445 (MU300_29440)	2818..3075	-	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
MU300_RS29450 (MU300_29445)	3085..4770	-	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
MU300_RS29455 (MU300_29450)	5097..5342	+	246	WP_032440458.1	hypothetical protein	-
MU300_RS29460 (MU300_29455)	5616..5987	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
MU300_RS29465 (MU300_29460)	5984..6349	+	366	WP_072354022.1	TonB family protein	-
MU300_RS29470 (MU300_29465)	6524..6802	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
MU300_RS29475 (MU300_29470)	6790..7104	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MU300_RS29480 (MU300_29475)	7268..7696	+	429	WP_001140599.1	hypothetical protein	-
MU300_RS29485 (MU300_29480)	7722..7901	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
MU300_RS29490 (MU300_29485)	7928..8458	-	531	WP_137970705.1	hypothetical protein	-
MU300_RS29495 (MU300_29490)	8465..9196	-	732	WP_071177728.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T241751 WP_071177730.1 NZ_CP095246:6790-7104 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T241751 NZ_OU015340:3642573-3642848 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGGGCAAAGGGAAATTGAATATTCGGGACAGTTCCAGAAAGATGTAAAACGGGCACAAAAGCGTCATAAGGATGTCGG
CAAACTTAAAACACTTATGACGCTTCTGATTCACCACCCTTTTCCCCTTCCTGCCATTTATAAAGATCACCCGTTACAAG
GTTCATATAGCGGATACCGGGATGCCCATATTGAGCCTGACTGGATTCTTATTTATAAGATAACGGATGAATGCCTGCGA
TTTGAACGAACAGGAACGCATGCCGATTTATTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT241751 WP_000093040.1 NZ_CP095246:6524-6802 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT241751 NZ_OU015340:3642310-3642570 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGCTGCAAATGCGCTTGTTCGTGCCCGTATCGACGAAACGCTGAAGGACCAGGCGGCAGATGTCCTGGCTGAAATGGG
GTTAACCATTTCTGATTTGATACGGATTACACTGACTAAAGTTGCGCGTGAAAAGGCATTGCCTTTTGATTTGCGCATAC
CCAATGAGTTAACGTCCCGAACGATAGAAAATAGCGAAGCTGGCGTTGATATTCATAAAGCCAAAGATGCTGATGATTTG
TTTGACCAGTTAGGGATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1