Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3695322..3696132 Replicon chromosome
Accession NZ_CP095240
Organism Klebsiella pneumoniae strain 21080237

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag MU300_RS18195 Protein ID WP_002887280.1
Coordinates 3695599..3696132 (+) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag MU300_RS18190 Protein ID WP_002887278.1
Coordinates 3695322..3695588 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU300_RS18170 (MU300_18165) 3691194..3691538 - 345 WP_002887266.1 cation efflux system protein CusF -
MU300_RS18175 (MU300_18170) 3691556..3692941 - 1386 WP_002887267.1 efflux transporter outer membrane subunit -
MU300_RS18180 (MU300_18175) 3693113..3693796 + 684 WP_002887273.1 copper response regulator transcription factor CusR -
MU300_RS18185 (MU300_18180) 3693786..3695219 + 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
MU300_RS18190 (MU300_18185) 3695322..3695588 + 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
MU300_RS18195 (MU300_18190) 3695599..3696132 + 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
MU300_RS18200 (MU300_18195) 3696180..3697301 - 1122 WP_002887282.1 cupin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T241742 WP_002887280.1 NZ_CP095240:3695599-3696132 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T241742 NZ_OU015340:1750947-1751165 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT241742 WP_002887278.1 NZ_CP095240:3695322-3695588 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT241742 NZ_OU015340:1750545-1750919 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References