Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3112629..3113254 Replicon chromosome
Accession NZ_CP095240
Organism Klebsiella pneumoniae strain 21080237

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU300_RS15410 Protein ID WP_002882817.1
Coordinates 3112871..3113254 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU300_RS15405 Protein ID WP_004150355.1
Coordinates 3112629..3112871 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU300_RS15380 (MU300_15375) 3108620..3109219 + 600 WP_004151865.1 glucose-1-phosphatase -
MU300_RS15385 (MU300_15380) 3109213..3110073 + 861 WP_002882807.1 virulence factor BrkB family protein -
MU300_RS15390 (MU300_15385) 3110070..3110507 + 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU300_RS15395 (MU300_15390) 3110552..3111493 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU300_RS15400 (MU300_15395) 3111507..3112424 - 918 WP_002882812.1 alpha/beta hydrolase -
MU300_RS15405 (MU300_15400) 3112629..3112871 + 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU300_RS15410 (MU300_15405) 3112871..3113254 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU300_RS15415 (MU300_15410) 3113428..3114357 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU300_RS15420 (MU300_15415) 3114354..3114989 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU300_RS15425 (MU300_15420) 3114986..3115888 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241739 WP_002882817.1 NZ_CP095240:3112871-3113254 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241739 NZ_OU015323:c4282378-4281851 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241739 WP_004150355.1 NZ_CP095240:3112629-3112871 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241739 NZ_OU015323:c4282665-4282375 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References