Detailed information of TA system TA241724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 2613..3193
• Replicon: plasmid p21072329_4
• Accession: NZ_CP095238
• Organism: Klebsiella pneumoniae strain 21072329

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: MU289_RS28770
• Protein ID: WP_071177730.1
• Coordinates: 2613..2927 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: MU289_RS28775
• Protein ID: WP_000093040.1
• Coordinates: 2915..3193 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MU289_RS28750 (MU289_28750)	521..1252	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
MU289_RS28755 (MU289_28755)	1259..1789	+	531	WP_071177729.1	hypothetical protein	-
MU289_RS28760 (MU289_28760)	1816..1995	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
MU289_RS28765 (MU289_28765)	2021..2449	-	429	WP_001140599.1	hypothetical protein	-
MU289_RS28770 (MU289_28770)	2613..2927	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MU289_RS28775 (MU289_28775)	2915..3193	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
MU289_RS28780 (MU289_28780)	3368..3733	-	366	WP_072354022.1	TonB family protein	-
MU289_RS28785 (MU289_28785)	3730..4101	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
MU289_RS28790 (MU289_28790)	4375..4620	-	246	WP_032440458.1	hypothetical protein	-
MU289_RS28795 (MU289_28795)	4947..6632	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
MU289_RS28800 (MU289_28800)	6642..6899	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
MU289_RS28805 (MU289_28805)	6984..7133	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T241724 WP_071177730.1 NZ_CP095238:c2927-2613 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T241724 NZ_OU015319:c2454021-2453722 [Cloacibacterium caeni]
ATGAATTACAAAATTAGCATAGAAGCCGAAAAAGATTTAGAAAATATTTGGCTTTATACACTAGAAAATTGGTCTATAGA
ACAAGCAGATTACTATTTTGATTTAATAATGAATGAAATAGAATATCTTGCAGAAAATCCTAAATTCGGACAAGATTTCG
GGGAAGTTAGAAAAGGTTATTTTCGCTCTCGTGTAAAATCCCATTTTATTTTTTACAGAATTAATCTCAAAAATAACGAT
ATAGAAATCATACGAATCTTGCATCAACAAATGGATGTAGATTCTCATTTAAATGTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT241724 WP_000093040.1 NZ_CP095238:c3193-2915 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT241724 NZ_OU015319:c2454269-2454024 [Cloacibacterium caeni]
ATGGCAAAAAATACTTCTATTTTGCTCGGAGAATATTTTGAGAATTTCATTTCAGAACAAATTAAGTCTGGTAAATTTTC
TTCTGCTAGTGAATTAATTAGAACAGCTTTAAGGGTTTTTGAACATCAAGAATCTCAAAAAACATTATTAATTAACGAAC
TAAAAAAAGGAGAAAAATCTGGCTTTTCAAAAGATTTTAATCGAGATTCTTTTAAAGAAAAGCTTCACAAAAAACATGCT
GAATAG

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1