Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 134199..134842 Replicon plasmid p21072329_1
Accession NZ_CP095235
Organism Klebsiella pneumoniae strain 21072329

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag MU289_RS27740 Protein ID WP_001044770.1
Coordinates 134199..134615 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag MU289_RS27745 Protein ID WP_001261282.1
Coordinates 134612..134842 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU289_RS27720 (130302) 130302..130574 - 273 Protein_169 transposase -
MU289_RS27730 (131556) 131556..132578 - 1023 WP_000361404.1 helicase UvrD -
MU289_RS27735 (132563) 132563..134125 - 1563 WP_004206609.1 AAA family ATPase -
MU289_RS27740 (134199) 134199..134615 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
MU289_RS27745 (134612) 134612..134842 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MU289_RS27750 (134799) 134799..135260 + 462 WP_014343465.1 hypothetical protein -
MU289_RS27755 (135421) 135421..136365 + 945 WP_011977810.1 hypothetical protein -
MU289_RS27760 (136402) 136402..136794 + 393 WP_011977811.1 hypothetical protein -
MU289_RS27765 (136852) 136852..137373 + 522 WP_094960442.1 hypothetical protein -
MU289_RS27770 (137419) 137419..137622 + 204 WP_011977813.1 hypothetical protein -
MU289_RS27775 (137652) 137652..138656 + 1005 WP_011977814.1 hypothetical protein -
MU289_RS27780 (138840) 138840..139619 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid catA2 / tet(A) / dfrA1 / qacE / sul1 / mph(A) / aph(3')-Ia / blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 - 1..173108 173108


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T241721 WP_001044770.1 NZ_CP095235:c134615-134199 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T241721 NZ_OD940440:2717900-2718040 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT241721 WP_001261282.1 NZ_CP095235:c134842-134612 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT241721 NZ_OD940440:c2718171-2717973 [Enterococcus faecalis]
TTTTTAGGTAATTGTGCTATAATGGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References