Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2273273..2273898 Replicon chromosome
Accession NZ_CP095234
Organism Klebsiella pneumoniae strain 21072329

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag MU289_RS11015 Protein ID WP_002882817.1
Coordinates 2273273..2273656 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag MU289_RS11020 Protein ID WP_004150355.1
Coordinates 2273656..2273898 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MU289_RS11000 (MU289_11000) 2270639..2271541 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
MU289_RS11005 (MU289_11005) 2271538..2272173 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
MU289_RS11010 (MU289_11010) 2272170..2273099 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
MU289_RS11015 (MU289_11015) 2273273..2273656 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
MU289_RS11020 (MU289_11020) 2273656..2273898 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
MU289_RS11025 (MU289_11025) 2274103..2275020 + 918 WP_002882812.1 alpha/beta hydrolase -
MU289_RS11030 (MU289_11030) 2275034..2275975 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
MU289_RS11035 (MU289_11035) 2276020..2276457 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
MU289_RS11040 (MU289_11040) 2276454..2277314 - 861 WP_002882807.1 virulence factor BrkB family protein -
MU289_RS11045 (MU289_11045) 2277308..2277907 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T241705 WP_002882817.1 NZ_CP095234:c2273656-2273273 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T241705 NZ_OD940437:c305343-305248 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCGATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT241705 WP_004150355.1 NZ_CP095234:c2273898-2273656 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT241705 NZ_OD940437:305148-305213 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References