Detailed information of TA system TA024170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 2068240..2068775
• Replicon: chromosome
• Accession: NC_012988
• Organism: Methylobacterium extorquens DM4 (Methylobacterium)

Toxin (Protein)

• Gene name: parE
• Uniprot ID: C7C703
• Locus tag: METD_RS09540
• Protein ID: WP_015822130.1
• Coordinates: 2068485..2068775 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: METD_RS09535
• Protein ID: WP_012253127.1
• Coordinates: 2068240..2068488 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METD_RS09520	2063458..2065041	-	1584	WP_015822127.1	hypothetical protein	-
METD_RS09525	2065041..2066408	-	1368	WP_015822128.1	SWIM zinc finger family protein	-
METD_RS09530	2066604..2068106	-	1503	WP_015822129.1	glucose-6-phosphate dehydrogenase	-
METD_RS09535	2068240..2068488	+	249	WP_012253127.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
METD_RS09540	2068485..2068775	+	291	WP_015822130.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
METD_RS09545	2068857..2069495	+	639	WP_015822131.1	uracil-DNA glycosylase family protein	-
METD_RS09550	2069608..2071133	-	1526	Protein_1922	alginate export family protein	-
METD_RS09555	2071458..2072156	+	699	WP_015822132.1	orotidine-5'-phosphate decarboxylase	-
METD_RS09560	2072159..2072503	+	345	WP_003605408.1	DUF1330 domain-containing protein	-
METD_RS09565	2072523..2073146	-	624	WP_012253132.1	TetR/AcrR family transcriptional regulator	-
METD_RS09570	2073369..2073569	-	201	WP_003605412.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11059.61 Da
• Isoelectric Point: 10.5105

>T24170 WP_015822130.1 NC_012988:2068485-2068775 [Methylorubrum extorquens AM1]
MSVRLSPRARADLSRIWDDSAERWGADQADRYIRLLAGGFDRLAEDPARGLRADEIRKGYFRLSVGSHVLFYRLGAEGGI
EVIRILHGRMDFKRHF

Nucleotide

• Length: 291 bp

>T24170 NC_012988:2068485-2068775 [Methylorubrum extorquens AM1]
ATGAGCGTCCGCCTCTCGCCGAGGGCGCGCGCGGATCTCAGCCGGATCTGGGACGATTCGGCCGAGCGCTGGGGAGCGGA
TCAGGCCGACCGCTACATCCGCCTTCTGGCTGGAGGCTTCGACCGATTGGCGGAGGATCCGGCTCGGGGTCTAAGGGCCG
ATGAGATCCGCAAGGGGTATTTCCGCCTCTCCGTCGGATCGCACGTCCTGTTTTACCGCCTGGGCGCCGAGGGCGGCATT
GAGGTCATCCGCATCCTTCACGGGCGGATGGATTTCAAACGTCACTTTTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8974.00 Da
• Isoelectric Point: 4.5173

>AT24170 WP_012253127.1 NC_012988:2068240-2068488 [Methylorubrum extorquens AM1]
MPRNTSVSLGEHFAQFIDRQVEGGRYGSASEVVRAGLRLLEEQEAALDTLRAALIEGEESGPPELFDTEAFLREKRAGAI
RG

Nucleotide

• Length: 249 bp

>AT24170 NC_012988:2068240-2068488 [Methylorubrum extorquens AM1]
ATGCCGCGAAACACATCCGTCTCGCTCGGCGAGCACTTTGCGCAGTTCATCGACCGCCAGGTCGAGGGGGGACGTTACGG
CTCCGCCAGCGAAGTCGTCCGTGCCGGTCTTCGTCTCCTTGAGGAGCAGGAGGCCGCCCTCGATACGCTCCGGGCCGCGC
TGATCGAGGGGGAGGAGAGCGGCCCGCCCGAGCTCTTCGATACGGAGGCCTTTCTCCGGGAGAAGCGTGCGGGCGCGATC
CGAGGATGA

Structures

Toxin

• AlphaFold DB: C7C703