Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 141924..142567 Replicon plasmid pEk140_mcr-10
Accession NZ_CP095181
Organism Enterobacter roggenkampii strain Ek140

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag MUY32_RS24575 Protein ID WP_000754567.1
Coordinates 141924..142340 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag MUY32_RS24580 Protein ID WP_023293777.1
Coordinates 142337..142567 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY32_RS24545 (MUY32_24545) 137110..137691 + 582 WP_008502230.1 TetR/AcrR family transcriptional regulator -
MUY32_RS24550 (MUY32_24550) 137696..138034 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
MUY32_RS24555 (MUY32_24555) 138064..138393 - 330 WP_008502229.1 thioredoxin family protein -
MUY32_RS24560 (MUY32_24560) 138606..139712 + 1107 WP_006687059.1 alkene reductase -
MUY32_RS24565 (MUY32_24565) 139778..140479 + 702 WP_008502228.1 DsbA family oxidoreductase -
MUY32_RS24570 (MUY32_24570) 140605..141573 + 969 WP_084832609.1 IS5-like element IS903B family transposase -
MUY32_RS24575 (MUY32_24575) 141924..142340 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
MUY32_RS24580 (MUY32_24580) 142337..142567 - 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUY32_RS24585 (MUY32_24585) 142929..144076 + 1148 WP_085949497.1 IS3 family transposase -
MUY32_RS24590 (MUY32_24590) 144078..144371 - 294 WP_249494757.1 hypothetical protein -
MUY32_RS24595 (MUY32_24595) 144507..144578 - 72 Protein_151 hypothetical protein -
MUY32_RS24600 (MUY32_24600) 144708..145913 + 1206 WP_006797591.1 AAA family ATPase -
MUY32_RS24605 (MUY32_24605) 145913..146887 + 975 WP_000064119.1 ParB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 - 1..201356 201356
- inside IScluster/Tn - - 124391..144076 19685


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T241696 WP_000754567.1 NZ_CP095181:c142340-141924 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T241696 NZ_OD940433:49482-49592 [Enterococcus faecalis]
GTGTTATTTGTGAAAGATTTAATGTCGTTGGTTATCGCACCGATCTTTGTAGGATTGGTTCTGGAAATGATTTCTCGTGT
GTTGGACGAGGAAGACGATAACCGAAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT241696 WP_023293777.1 NZ_CP095181:c142567-142337 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT241696 NZ_OD940433:c49696-49632 [Enterococcus faecalis]
AACGACATTAAATCGTACAAATAACACAAAAAGCAATCCTACGGCGAATAGGATTGCTTTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References