Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 76086..76822 Replicon plasmid pEk140_mcr-10
Accession NZ_CP095181
Organism Enterobacter roggenkampii strain Ek140

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MUY32_RS24265 Protein ID WP_003026803.1
Coordinates 76340..76822 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MUY32_RS24260 Protein ID WP_003026799.1
Coordinates 76086..76352 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY32_RS24235 (MUY32_24235) 71553..72293 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
MUY32_RS24240 (MUY32_24240) 72360..73580 - 1221 WP_000343760.1 ISL3-like element ISKox3 family transposase -
MUY32_RS24245 (MUY32_24245) 73981..74949 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
MUY32_RS24250 (MUY32_24250) 75194..75397 + 204 WP_004213596.1 HHA domain-containing protein -
MUY32_RS24255 (MUY32_24255) 75411..75641 + 231 WP_023307222.1 hypothetical protein -
MUY32_RS24260 (MUY32_24260) 76086..76352 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MUY32_RS24265 (MUY32_24265) 76340..76822 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MUY32_RS24270 (MUY32_24270) 77034..78377 + 1344 WP_080339306.1 ISNCY family transposase -
MUY32_RS24275 (MUY32_24275) 78448..79266 - 819 WP_023307220.1 abortive infection family protein -
MUY32_RS24280 (MUY32_24280) 79275..79847 - 573 WP_032676095.1 recombinase family protein -
MUY32_RS24285 (MUY32_24285) 80299..81279 + 981 WP_000082736.1 IS5-like element ISEc68 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-10 - 1..201356 201356
- inside IScluster/Tn - - 72360..96071 23711


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T241695 WP_003026803.1 NZ_CP095181:76340-76822 [Enterobacter roggenkampii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T241695 NZ_OD940432:c70277-69414 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGATCAGGTAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAGACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAATACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
AGATAAAGGGTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGATTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGGTATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGGATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT241695 WP_003026799.1 NZ_CP095181:76086-76352 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT241695 NZ_OD940432:c70551-70279 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References