Detailed information of TA system TA241694

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4205718..4206397
• Replicon: chromosome
• Accession: NZ_CP095180
• Organism: Enterobacter roggenkampii strain Ek140

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: MUY32_RS20245
• Protein ID: WP_232926835.1
• Coordinates: 4205718..4206059 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: MUY32_RS20250
• Protein ID: WP_097724915.1
• Coordinates: 4206080..4206397 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY32_RS20225 (MUY32_20225)	4202022..4203869	+	1848	WP_008503184.1	RNA polymerase sigma factor RpoD	-
MUY32_RS20230 (MUY32_20230)	4203960..4204466	-	507	WP_008503183.1	G/U mismatch-specific DNA glycosylase	-
MUY32_RS20240 (MUY32_20240)	4204769..4205602	-	834	WP_046495848.1	DUF4942 domain-containing protein	-
MUY32_RS20245 (MUY32_20245)	4205718..4206059	-	342	WP_232926835.1	TA system toxin CbtA family protein	Toxin
MUY32_RS20250 (MUY32_20250)	4206080..4206397	-	318	WP_097724915.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MUY32_RS20255 (MUY32_20255)	4206416..4206637	-	222	WP_000691994.1	DUF987 domain-containing protein	-
MUY32_RS20260 (MUY32_20260)	4206646..4207122	-	477	WP_000811693.1	RadC family protein	-
MUY32_RS20265 (MUY32_20265)	4207138..4207596	-	459	WP_000211838.1	antirestriction protein	-
MUY32_RS20270 (MUY32_20270)	4207694..4207933	-	240	WP_097725009.1	DUF905 domain-containing protein	-
MUY32_RS20275 (MUY32_20275)	4208010..4208477	-	468	WP_001547765.1	protein YkfB	-
MUY32_RS20280 (MUY32_20280)	4208500..4208943	-	444	WP_001547764.1	lipoprotein YafY	-
MUY32_RS20285 (MUY32_20285)	4208943..4209179	-	237	WP_001144031.1	protein YpjK	-
MUY32_RS20290 (MUY32_20290)	4209220..4209921	-	702	WP_232926834.1	WYL domain-containing protein	-
MUY32_RS20295 (MUY32_20295)	4210139..4210960	-	822	WP_001548934.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13003.05 Da
• Isoelectric Point: 10.2764

>T241694 WP_232926835.1 NZ_CP095180:c4206059-4205718 [Enterobacter roggenkampii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLKQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T241694 NZ_OD940432:c42366-42025 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11882.45 Da
• Isoelectric Point: 6.6246

>AT241694 WP_097724915.1 NZ_CP095180:c4206397-4206080 [Enterobacter roggenkampii]
MSNITWGLQRAITPRLGARLVQEGNRLHYLADRASITGKFSDVECRKLDETFPHFISQMESMLTTDELNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT241694 NZ_OD940432:c42596-42366 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA