Detailed information of TA system TA241692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3095404..3095994
• Replicon: chromosome
• Accession: NZ_CP095180
• Organism: Enterobacter roggenkampii strain Ek140

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: MUY32_RS15040
• Protein ID: WP_248274850.1
• Coordinates: 3095404..3095736 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MUY32_RS15045
• Protein ID: WP_248274851.1
• Coordinates: 3095737..3095994 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY32_RS15035 (MUY32_15035)	3091126..3095160	+	4035	WP_249456415.1	hypothetical protein	-
MUY32_RS15040 (MUY32_15040)	3095404..3095736	-	333	WP_248274850.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MUY32_RS15045 (MUY32_15045)	3095737..3095994	-	258	WP_248274851.1	antitoxin	Antitoxin
MUY32_RS15050 (MUY32_15050)	3096274..3096663	-	390	WP_094943150.1	RidA family protein	-
MUY32_RS15055 (MUY32_15055)	3096805..3097743	+	939	WP_039024970.1	LysR family transcriptional regulator	-
MUY32_RS15060 (MUY32_15060)	3097752..3098352	-	601	Protein_2955	LysR family transcriptional regulator	-
MUY32_RS15065 (MUY32_15065)	3098523..3099131	+	609	WP_206805453.1	glutathione S-transferase family protein	-
MUY32_RS15070 (MUY32_15070)	3099175..3100125	-	951	WP_248274852.1	HTH-type transcriptional regulator Cbl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11827.77 Da
• Isoelectric Point: 10.7301

>T241692 WP_248274850.1 NZ_CP095180:c3095736-3095404 [Enterobacter roggenkampii]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEGAGTKTMGVIRCDQPRTI
DMAARNGMRLERIPDAVVNEVLARLNAILS

Nucleotide

• Length: 333 bp

>T241692 NZ_OD940431:c2701611-2701270 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGCAGAGACGCCTTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGTCATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9440.92 Da
• Isoelectric Point: 4.4607

>AT241692 WP_248274851.1 NZ_CP095180:c3095994-3095737 [Enterobacter roggenkampii]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWMDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT241692 NZ_OD940431:c2701841-2701611 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA