Detailed information of TA system TA241683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 1744608..1745405
• Replicon: chromosome
• Accession: NZ_CP095180
• Organism: Enterobacter roggenkampii strain Ek140

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: MUY32_RS08235
• Protein ID: WP_248275328.1
• Coordinates: 1744884..1745405 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: MUY32_RS08230
• Protein ID: WP_008499829.1
• Coordinates: 1744608..1744877 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY32_RS08200 (MUY32_08200)	1740230..1740673	-	444	WP_008499834.1	helix-turn-helix domain-containing protein	-
MUY32_RS08205 (MUY32_08205)	1740826..1742067	+	1242	WP_008499833.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
MUY32_RS08210 (MUY32_08210)	1742103..1742330	-	228	WP_008499832.1	YccJ family protein	-
MUY32_RS08215 (MUY32_08215)	1742351..1742947	-	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
MUY32_RS08220 (MUY32_08220)	1743339..1743509	+	171	WP_013097201.1	general stress protein	-
MUY32_RS08225 (MUY32_08225)	1743629..1744546	+	918	WP_249494610.1	DMT family transporter	-
MUY32_RS08230 (MUY32_08230)	1744608..1744877	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
MUY32_RS08235 (MUY32_08235)	1744884..1745405	+	522	WP_248275328.1	GNAT family N-acetyltransferase	Toxin
MUY32_RS08240 (MUY32_08240)	1745479..1746801	-	1323	WP_045352935.1	pyrimidine utilization transport protein G	-
MUY32_RS08245 (MUY32_08245)	1746823..1747317	-	495	WP_023292823.1	pyrimidine utilization flavin reductase protein F	-
MUY32_RS08250 (MUY32_08250)	1747327..1747917	-	591	WP_032651257.1	malonic semialdehyde reductase	-
MUY32_RS08255 (MUY32_08255)	1747927..1748727	-	801	WP_021240807.1	pyrimidine utilization protein D	-
MUY32_RS08260 (MUY32_08260)	1748735..1749121	-	387	WP_008499822.1	pyrimidine utilization protein C	-
MUY32_RS08265 (MUY32_08265)	1749133..1749822	-	690	WP_248275329.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19627.39 Da
• Isoelectric Point: 7.9331

>T241683 WP_248275328.1 NZ_CP095180:1744884-1745405 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYNFRDFDCGEPSLNVFLTEHLARQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTGENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T241683 NZ_OD940422:1172301-1172444 [Enterococcus faecalis]
ATGAATGTAAGTGCTAAAATCTATGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT241683 WP_008499829.1 NZ_CP095180:1744608-1744877 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT241683 NZ_OD940422:c1172635-1172377 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTTATTGATGGTCTTTTTAGGGTTGTTGTGCTATAA
TGATAAAGAAAAGAGAGATATGCTTCCAACATATCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATTTTATTATTT
AAAAATAACCGTACTTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCAATCAAA
GCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: W7PG43