Detailed information of TA system TA024168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 220732..221314
• Replicon: chromosome
• Accession: NC_012988
• Organism: Methylobacterium extorquens DM4 (Methylobacterium)

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: C7C6Y0
• Locus tag: METD_RS01025
• Protein ID: WP_012779030.1
• Coordinates: 221039..221314 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: METD_RS01020
• Protein ID: WP_041357242.1
• Coordinates: 220732..221049 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
METD_RS01005	216003..216977	-	975	WP_012779026.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
METD_RS01010	217137..219923	+	2787	WP_012779027.1	[protein-PII] uridylyltransferase	-
METD_RS01015	220015..220623	+	609	WP_012779028.1	nucleotide exchange factor GrpE	-
METD_RS01020	220732..221049	-	318	WP_041357242.1	BrnA antitoxin family protein	Antitoxin
METD_RS01025	221039..221314	-	276	WP_012779030.1	BrnT family toxin	Toxin
METD_RS01030	221406..222263	-	858	WP_012779031.1	prephenate dehydratase	-
METD_RS01035	222280..223023	-	744	WP_003600755.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
METD_RS28520	223331..224248	+	918	WP_012779032.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10005.49 Da
• Isoelectric Point: 5.8151

>T24168 WP_012779030.1 NC_012988:c221314-221039 [Methylorubrum extorquens AM1]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVAVAQLGATGSARMKIIGEFDGQTVVAAIIAPLGQEAISLISLRRA
SRSERRLYDAR

Nucleotide

• Length: 276 bp

>T24168 NC_012988:c221314-221039 [Methylorubrum extorquens AM1]
ATGTTGATTGTCTGGGACGAGCCCAAGCGCCTGACCAACCTGCAAAAGCACGGTCTCGACTTCGCGGATTTCGAAGCGGG
CTTCGACTTCGAGACCGCTCTTGTCGCGGTTGCCCAACTAGGCGCTACCGGCTCAGCCCGAATGAAGATCATCGGCGAGT
TCGACGGACAGACGGTGGTGGCAGCGATCATCGCTCCCTTGGGACAGGAAGCGATCTCCCTCATCAGCCTGCGCCGAGCC
AGCCGGAGTGAACGGAGGCTCTACGATGCCCGCTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11607.02 Da
• Isoelectric Point: 5.1634

>AT24168 WP_041357242.1 NC_012988:c221049-220732 [Methylorubrum extorquens AM1]
MPADKPDRTPITTSEEAGIQAGIAADPDNPEWTEADFRSAVPFEQAFPVLAEKLRRTRGPQSAPTKQLVSLRLDHDVLLR
FRATGPGWQSRMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT24168 NC_012988:c221049-220732 [Methylorubrum extorquens AM1]
ATGCCCGCTGATAAGCCCGATCGGACACCGATCACGACCTCGGAGGAGGCCGGGATCCAGGCCGGCATCGCGGCCGATCC
CGACAATCCGGAATGGACCGAAGCGGACTTTCGAAGCGCCGTGCCCTTCGAACAGGCCTTTCCCGTCCTCGCAGAGAAAC
TGCGCCGCACCCGCGGCCCCCAGAGCGCTCCGACCAAACAGCTCGTCAGCCTGAGGCTCGATCACGACGTCCTGCTTCGT
TTCAGGGCGACCGGTCCGGGCTGGCAGTCTCGGATGAATGAAGCCCTGCGCCGGGCGGCGGCCGATCTCCCCGACTGA

Structures

Toxin

• AlphaFold DB: C7C6Y0