Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 130775..131511 Replicon plasmid pK528_mcr-10
Accession NZ_CP095178
Organism Enterobacter hormaechei strain K528

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MUY31_RS23290 Protein ID WP_003026803.1
Coordinates 131029..131511 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MUY31_RS23285 Protein ID WP_003026799.1
Coordinates 130775..131041 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY31_RS23260 (MUY31_23260) 126488..127228 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
MUY31_RS23265 (MUY31_23265) 127595..128563 - 969 WP_023307223.1 IS5-like element IS903B family transposase -
MUY31_RS23270 (MUY31_23270) 128890..129870 + 981 WP_023292560.1 IS5-like element ISKpn26 family transposase -
MUY31_RS23275 (MUY31_23275) 130033..130086 + 54 WP_227526284.1 hypothetical protein -
MUY31_RS23280 (MUY31_23280) 130100..130330 + 231 WP_023307222.1 hypothetical protein -
MUY31_RS23285 (MUY31_23285) 130775..131041 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MUY31_RS23290 (MUY31_23290) 131029..131511 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MUY31_RS23295 (MUY31_23295) 131720..133066 + 1347 WP_076611902.1 ISNCY-like element ISLad2 family transposase -
MUY31_RS23300 (MUY31_23300) 133125..133928 + 804 WP_000589340.1 hypothetical protein -
MUY31_RS23305 (MUY31_23305) 133942..135303 + 1362 WP_000482601.1 FRG domain-containing protein -
MUY31_RS23310 (MUY31_23310) 135456..135896 + 441 WP_016239970.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid catA1 / ant(3'')-Ia / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaSHV-12 / qnrB1 / dfrA14 / tet(A) / mcr-10 mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..148937 148937
- inside IScluster/Tn - - 127595..138418 10823


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T241679 WP_003026803.1 NZ_CP095178:131029-131511 [Enterobacter hormaechei]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T241679 NZ_OD940422:c1027078-1026977 [Enterococcus faecalis]
TTGTCAATCGAAGCGACATTGGAATTGATGATTAGTTTTGCAACCCTTGTTGCGTTACTGATTTTCGATATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT241679 WP_003026799.1 NZ_CP095178:130775-131041 [Enterobacter hormaechei]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT241679 NZ_OD940422:1026855-1027040 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCCTTTATACCAACGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATATCGA
AAATCAGTAACGCAACAAGGGTTGCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References