Detailed information of TA system TA241675

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ypjF-yeeU/CbtA-CbeA
• Location: 3515367..3516100
• Replicon: chromosome
• Accession: NZ_CP095177
• Organism: Enterobacter hormaechei strain K528

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: MUY31_RS16995
• Protein ID: WP_181041938.1
• Coordinates: 3515771..3516100 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MUY31_RS16990
• Protein ID: WP_134871852.1
• Coordinates: 3515367..3515765 (+)
• Length: 133 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY31_RS16955 (MUY31_16955)	3511276..3511710	+	435	WP_109948925.1	hypothetical protein	-
MUY31_RS16960 (MUY31_16960)	3511770..3512609	+	840	WP_249464999.1	hypothetical protein	-
MUY31_RS16965 (MUY31_16965)	3512668..3512859	+	192	WP_041412185.1	hypothetical protein	-
MUY31_RS16970 (MUY31_16970)	3513273..3514094	+	822	WP_213317511.1	DUF932 domain-containing protein	-
MUY31_RS16975 (MUY31_16975)	3514125..3514568	+	444	WP_181041937.1	antirestriction protein	-
MUY31_RS16980 (MUY31_16980)	3514581..3515123	+	543	WP_249465001.1	DNA repair protein RadC	-
MUY31_RS16985 (MUY31_16985)	3515120..3515341	+	222	WP_181041939.1	DUF987 domain-containing protein	-
MUY31_RS16990 (MUY31_16990)	3515367..3515765	+	399	WP_134871852.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MUY31_RS16995 (MUY31_16995)	3515771..3516100	+	330	WP_181041938.1	TA system toxin CbtA family protein	Toxin
MUY31_RS17000 (MUY31_17000)	3516375..3517685	-	1311	WP_048241969.1	amidase family protein	-
MUY31_RS17005 (MUY31_17005)	3517740..3519035	-	1296	WP_022651679.1	histidinol dehydrogenase	-
MUY31_RS17010 (MUY31_17010)	3519036..3520178	-	1143	WP_003862281.1	ester cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3499574..3516260	16686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12412.12 Da
• Isoelectric Point: 6.8685

>T241675 WP_181041938.1 NZ_CP095177:3515771-3516100 [Enterobacter hormaechei]
MQTISSHPTRATQSCLSPVEIWQLLLTHLLSQHYGLTLNDTPFNDETTIQEHIDAGISLSDAVNFLVEKYGLVRIDRKGF
SWQEQTPYLSVVDILRARRTTSLLKTNVK

Nucleotide

• Length: 330 bp

>T241675 NZ_OD940420:c2801168-2801025 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATCCTAGGTTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 133 a.a.
• Molecular weight: 14705.61 Da
• Isoelectric Point: 5.5048

>AT241675 WP_134871852.1 NZ_CP095177:3515367-3515765 [Enterobacter hormaechei]
MNNHSESGTKPENPAIQQWGLKRDITPCFGARLVQEGNRLHYLADRAGFSGACTNDVAMRLDQAFPLMMKQLELMLTSGE
LNPRHAHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQYPPAYSLNISE

Nucleotide

• Length: 399 bp

>AT241675 NZ_OD940420:2801269-2801422 [Enterococcus faecalis]
TTTTTAGGGAATTGTGCTATAATAAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCGATTTTGTTACAAAAAATAACCGTACTCAGTCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAA