Detailed information of TA system TA241674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 3089902..3091685
• Replicon: chromosome
• Accession: NZ_CP095177
• Organism: Enterobacter hormaechei strain K528

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: MUY31_RS14985
• Protein ID: WP_045331150.1
• Coordinates: 3089902..3091251 (+)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: MUY31_RS14990
• Protein ID: WP_003859317.1
• Coordinates: 3091248..3091685 (+)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY31_RS14960 (MUY31_14960)	3085725..3086660	-	936	WP_003859306.1	tRNA dihydrouridine(16) synthase DusC	-
MUY31_RS14965 (MUY31_14965)	3086829..3087224	+	396	WP_003859308.1	CidA/LrgA family protein	-
MUY31_RS14970 (MUY31_14970)	3087221..3087916	+	696	WP_048240462.1	CidB/LrgB family autolysis modulator	-
MUY31_RS14975 (MUY31_14975)	3088045..3088929	+	885	WP_003859312.1	cytidine deaminase	-
MUY31_RS14980 (MUY31_14980)	3089057..3089767	+	711	WP_003859314.1	outer membrane permeability protein SanA	-
MUY31_RS14985 (MUY31_14985)	3089902..3091251	+	1350	WP_045331150.1	HipA domain-containing protein	Toxin
MUY31_RS14990 (MUY31_14990)	3091248..3091685	+	438	WP_003859317.1	helix-turn-helix transcriptional regulator	Antitoxin
MUY31_RS14995 (MUY31_14995)	3091724..3092734	-	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
MUY31_RS15000 (MUY31_15000)	3092750..3094270	-	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
MUY31_RS15005 (MUY31_15005)	3094350..3095348	-	999	WP_045337654.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
MUY31_RS15010 (MUY31_15010)	3095644..3096666	-	1023	WP_045337656.1	HTH-type transcriptional regulator GalS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51140.83 Da
• Isoelectric Point: 4.6112

>T241674 WP_045331150.1 NZ_CP095177:3089902-3091251 [Enterobacter hormaechei]
METLTVQAYLNETWTDIALIKYPGSEKNDWNITQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYQFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T241674 NZ_OD940420:c2801168-2801025 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAGACAATTCAGACGATCCTAGGTTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT241674 WP_003859317.1 NZ_CP095177:3091248-3091685 [Enterobacter hormaechei]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT241674 NZ_OD940420:2800906-2801092 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCAATT
GTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGTG
CAATCAAAGCAATGGTAAACATACCAA

Structures

Antitoxin

• AlphaFold DB: A0A837FFV9