Detailed information of TA system TA241662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 37320..38117
• Replicon: plasmid pK666_111k
• Accession: NZ_CP095172
• Organism: Enterobacter roggenkampii strain K666

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: MUY33_RS24850
• Protein ID: WP_249489062.1
• Coordinates: 37614..38117 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: MUY33_RS24845
• Protein ID: WP_016582626.1
• Coordinates: 37320..37589 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY33_RS24820 (MUY33_24820)	32939..33664	-	726	WP_249489054.1	hypothetical protein	-
MUY33_RS24825 (MUY33_24825)	33946..35001	+	1056	WP_001238819.1	hypothetical protein	-
MUY33_RS24830 (MUY33_24830)	35071..35826	-	756	WP_052930811.1	DUF2971 domain-containing protein	-
MUY33_RS24835 (MUY33_24835)	35875..36228	-	354	WP_162199642.1	hypothetical protein	-
MUY33_RS24840 (MUY33_24840)	36234..36899	-	666	WP_000161333.1	AAA family ATPase	-
MUY33_RS24845 (MUY33_24845)	37320..37589	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
MUY33_RS24850 (MUY33_24850)	37614..38117	+	504	WP_249489062.1	GNAT family N-acetyltransferase	Toxin
MUY33_RS24855 (MUY33_24855)	38146..38310	-	165	WP_249489055.1	hypothetical protein	-
MUY33_RS24860 (MUY33_24860)	38303..38554	-	252	WP_249489056.1	hypothetical protein	-
MUY33_RS24865 (MUY33_24865)	38556..39248	-	693	WP_249489057.1	hypothetical protein	-
MUY33_RS24870 (MUY33_24870)	39262..39585	-	324	WP_000064174.1	hypothetical protein	-
MUY33_RS24875 (MUY33_24875)	39699..42608	-	2910	WP_249489058.1	tail fiber domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111251	111251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (77-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18629.34 Da
• Isoelectric Point: 8.8869

>T241662 WP_249489062.1 NZ_CP095172:37614-38117 [Enterobacter roggenkampii]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVQQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYPD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T241662 NZ_NQJO01000001:990422-990658 [Erwinia amylovora]
ATAAGCTATGAACTGCTCTTCGATCCCTGGGCCTTAAAAGAGTGGAAAAAACTGGGCGCAACGGTTCGGGAACAGTTCAA
AAAGAAACTGGCGGAAGTGCTGGTAAACCTGCAGGTGGAATCAGCCAGGCTGAGAGATCACCCGGACTGCTGCAAAATAA
AGCTGAAATCATCGGGTTATCGTCTGGTATATCAGGTTCAGGATGAACAGCTGGTAATGTTGGTTATAAGCGTTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT241662 WP_016582626.1 NZ_CP095172:37320-37589 [Enterobacter roggenkampii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT241662 NZ_NQJO01000001:990205-990432 [Erwinia amylovora]
GTGCGGCTGGACGACAAACTGAAAAGCAAAGCCTGTGCAGTTCTGGATGAACTGGATGTATCGCCAACGGAGGCGGTCCG
GCTGTTGTTCCAGTATATAGCGGAAAATGAGCGCCTACCGCTGAAAGCGCTGACGGTAAGTGATGAAAAAGACGCGCTGC
TGAAAACGGTTCGCGAAAGGCTGGCATCGCTACAGAAGGGAATAAAGGTAACGCTGGATAAGCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3