Detailed information of TA system TA241648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1727919..1728716
• Replicon: chromosome
• Accession: NZ_CP095170
• Organism: Enterobacter roggenkampii strain K666

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A155TD66
• Locus tag: MUY33_RS08280
• Protein ID: WP_032651255.1
• Coordinates: 1728195..1728716 (+)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: MUY33_RS08275
• Protein ID: WP_008499829.1
• Coordinates: 1727919..1728188 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY33_RS08245 (MUY33_08245)	1723542..1723985	-	444	WP_008499834.1	helix-turn-helix domain-containing protein	-
MUY33_RS08250 (MUY33_08250)	1724138..1725379	+	1242	WP_032651252.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
MUY33_RS08255 (MUY33_08255)	1725415..1725642	-	228	WP_008499832.1	YccJ family protein	-
MUY33_RS08260 (MUY33_08260)	1725663..1726259	-	597	WP_008499831.1	NAD(P)H:quinone oxidoreductase	-
MUY33_RS08265 (MUY33_08265)	1726651..1726821	+	171	WP_013097201.1	general stress protein	-
MUY33_RS08270 (MUY33_08270)	1726940..1727857	+	918	WP_025912980.1	DMT family transporter	-
MUY33_RS08275 (MUY33_08275)	1727919..1728188	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
MUY33_RS08280 (MUY33_08280)	1728195..1728716	+	522	WP_032651255.1	GNAT family N-acetyltransferase	Toxin
MUY33_RS08285 (MUY33_08285)	1728790..1730112	-	1323	WP_008499826.1	pyrimidine utilization transport protein G	-
MUY33_RS08290 (MUY33_08290)	1730134..1730628	-	495	WP_008499825.1	pyrimidine utilization flavin reductase protein F	-
MUY33_RS08295 (MUY33_08295)	1730638..1731228	-	591	WP_032651257.1	malonic semialdehyde reductase	-
MUY33_RS08300 (MUY33_08300)	1731238..1732038	-	801	WP_021240807.1	pyrimidine utilization protein D	-
MUY33_RS08305 (MUY33_08305)	1732046..1732432	-	387	WP_008499822.1	pyrimidine utilization protein C	-
MUY33_RS08310 (MUY33_08310)	1732444..1733132	-	689	Protein_1616	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19714.46 Da
• Isoelectric Point: 7.0497

>T241648 WP_032651255.1 NZ_CP095170:1728195-1728716 [Enterobacter roggenkampii]
VDNLTIEMFSEGKDYDFRDFDCGEPSLNVFLTEHLVRQHNGRILRGYLLKDRDGPRVLGYYTLSGSCFEKAMLPSKTQQR
RIPYSNVPSVTLGRLAIHKDLQGLEWGTTLVTHAMRVVYLASQAVGVHGIFVDALNDQAKQFYLKQGFIPLTDENSHSLF
FPTKSIERLFEQA

Nucleotide

• Length: 522 bp

>T241648 NZ_LT996890:2074246-2074608 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT241648 WP_008499829.1 NZ_CP095170:1727919-1728188 [Enterobacter roggenkampii]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT241648 NZ_LT996890:2074079-2074249 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Toxin

• AlphaFold DB: A0A155TD66

Antitoxin

• AlphaFold DB: W7PG43