Detailed information of TA system TA241645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-MazE
• Location: 45744..46405
• Replicon: chromosome
• Accession: NZ_CP095170
• Organism: Enterobacter roggenkampii strain K666

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MUY33_RS00230
• Protein ID: WP_023293716.1
• Coordinates: 46007..46405 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A443U1A0
• Locus tag: MUY33_RS00225
• Protein ID: WP_032654366.1
• Coordinates: 45744..46010 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MUY33_RS00200 (MUY33_00200)	41808..43136	-	1329	Protein_39	PTS transporter subunit EIIC	-
MUY33_RS00205 (MUY33_00205)	43148..43462	-	315	WP_008500132.1	PTS sugar transporter subunit IIB	-
MUY33_RS00210 (MUY33_00210)	43756..44706	+	951	WP_023293714.1	LacI family DNA-binding transcriptional regulator	-
MUY33_RS00215 (MUY33_00215)	44757..45053	-	297	WP_032654360.1	YicS family protein	-
MUY33_RS00220 (MUY33_00220)	45195..45614	+	420	WP_032654363.1	GNAT family N-acetyltransferase	-
MUY33_RS00225 (MUY33_00225)	45744..46010	+	267	WP_032654366.1	virulence protein	Antitoxin
MUY33_RS00230 (MUY33_00230)	46007..46405	+	399	WP_023293716.1	type II toxin-antitoxin system VapC family toxin	Toxin
MUY33_RS00235 (MUY33_00235)	46545..47345	+	801	WP_032654369.1	lipoprotein NlpA	-
MUY33_RS00240 (MUY33_00240)	47468..48247	+	780	WP_032654371.1	MBL fold metallo-hydrolase	-
MUY33_RS00245 (MUY33_00245)	48274..49227	+	954	WP_032654373.1	helix-turn-helix domain-containing protein	-
MUY33_RS00250 (MUY33_00250)	49410..51107	+	1698	WP_229046295.1	ShlB/FhaC/HecB family hemolysin secretion/activation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14799.00 Da
• Isoelectric Point: 6.4779

>T241645 WP_023293716.1 NZ_CP095170:46007-46405 [Enterobacter roggenkampii]
MRHMLDTNIVSHLFKRHPEVVSRMTCLAPGDVCISSITEAELLYGADKKKSSRLKETIREFLNTITICDWDSDAAATYGE
LRATLEKKGKVMGDLDQLIAAHAISRGTTIVTNDRAFRMVQELAVEDWTTTL

Nucleotide

• Length: 399 bp

>T241645 NZ_LT996890:1740474-1740740 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10009.38 Da
• Isoelectric Point: 4.6310

>AT241645 WP_032654366.1 NZ_CP095170:45744-46010 [Enterobacter roggenkampii]
MERTAKLFKKGQNQAVMLPAEFAFDTESVYIRRDEEGNVVLVAKPVLIKSWDNFLRMLAETDVPDTFLSKEERNQSVTTR
DPLDGACS

Nucleotide

• Length: 267 bp

>AT241645 NZ_LT996890:1740223-1740474 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A443U1A0