Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 17115..17758 Replicon plasmid pK475-2_mcr-10
Accession NZ_CP095167
Organism Enterobacter roggenkampii strain K475-2

Toxin (Protein)


Gene name vagD Uniprot ID A0A155IU92
Locus tag MUY30_RS24560 Protein ID WP_000754567.1
Coordinates 17115..17531 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A155IUD1
Locus tag MUY30_RS24565 Protein ID WP_023293777.1
Coordinates 17528..17758 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY30_RS24530 (MUY30_24530) 12301..12882 + 582 WP_032676110.1 TetR/AcrR family transcriptional regulator -
MUY30_RS24535 (MUY30_24535) 12887..13225 + 339 WP_000888080.1 carboxymuconolactone decarboxylase family protein -
MUY30_RS24540 (MUY30_24540) 13255..13584 - 330 WP_008502229.1 thioredoxin family protein -
MUY30_RS24545 (MUY30_24545) 13797..14903 + 1107 WP_006687059.1 alkene reductase -
MUY30_RS24550 (MUY30_24550) 14969..15670 + 702 WP_008502228.1 DsbA family oxidoreductase -
MUY30_RS24555 (MUY30_24555) 15796..16764 + 969 WP_176311895.1 IS5-like element IS903B family transposase -
MUY30_RS24560 (MUY30_24560) 17115..17531 - 417 WP_000754567.1 type II toxin-antitoxin system VapC family toxin Toxin
MUY30_RS24565 (MUY30_24565) 17528..17758 - 231 WP_023293777.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
MUY30_RS24570 (MUY30_24570) 17896..18450 + 555 WP_161646454.1 hypothetical protein -
MUY30_RS24575 (MUY30_24575) 18538..18609 - 72 Protein_23 hypothetical protein -
MUY30_RS24580 (MUY30_24580) 18739..19944 + 1206 WP_006797591.1 AAA family ATPase -
MUY30_RS24585 (MUY30_24585) 19944..20918 + 975 WP_249469900.1 ParB family protein -
MUY30_RS24590 (MUY30_24590) 21000..22271 - 1272 WP_032627083.1 Y-family DNA polymerase -
MUY30_RS24595 (MUY30_24595) 22271..22702 - 432 WP_006796638.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..89168 89168
- inside IScluster/Tn - - 2813..16764 13951


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15108.61 Da        Isoelectric Point: 8.5403

>T241644 WP_000754567.1 NZ_CP095167:c17531-17115 [Enterobacter roggenkampii]
MKKTWMLDTNICSFIMREQPAAVLKRLEQVVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T241644 NZ_LT996890:1715236-1715343 [Staphylococcus aureus]
ATGTTCAATTTATTAATTGACATCATGACTTCAGCTTTAAGCGGCTGTCTTGTTGCGTTTTTTGCACATTGGTTACGAAC
GCGCAACAATAAAAAAGGTGACAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8706.73 Da        Isoelectric Point: 4.4299

>AT241644 WP_023293777.1 NZ_CP095167:c17758-17528 [Enterobacter roggenkampii]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAQREDVVSDEGRFDP

Download         Length: 231 bp

>AT241644 NZ_LT996890:c1715420-1715360 [Staphylococcus aureus]
TACATAATAAATTGAACATCTAAATACACCAAATCCCCTCACTACTGCCATAGTGAGGGGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A155IU92

Antitoxin

Source ID Structure
AlphaFold DB A0A155IUD1

References