Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 6170..6906 Replicon plasmid pK475-2_mcr-10
Accession NZ_CP095167
Organism Enterobacter roggenkampii strain K475-2

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag MUY30_RS24495 Protein ID WP_003026803.1
Coordinates 6424..6906 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag MUY30_RS24490 Protein ID WP_003026799.1
Coordinates 6170..6436 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MUY30_RS24465 (MUY30_24465) 1709..2449 - 741 WP_001515717.1 tyrosine-type recombinase/integrase -
MUY30_RS24470 (MUY30_24470) 2813..3781 - 969 WP_176311895.1 IS5-like element IS903B family transposase -
MUY30_RS24475 (MUY30_24475) 4026..4229 + 204 WP_004213596.1 HHA domain-containing protein -
MUY30_RS24480 (MUY30_24480) 4243..4473 + 231 WP_023307222.1 hypothetical protein -
MUY30_RS24490 (MUY30_24490) 6170..6436 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
MUY30_RS24495 (MUY30_24495) 6424..6906 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
MUY30_RS24500 (MUY30_24500) 7118..8461 + 1344 WP_080339306.1 ISNCY family transposase -
MUY30_RS24505 (MUY30_24505) 8532..9350 - 819 WP_023307220.1 abortive infection family protein -
MUY30_RS24510 (MUY30_24510) 9359..9931 - 573 WP_032676095.1 recombinase family protein -
MUY30_RS24515 (MUY30_24515) 10271..10975 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
MUY30_RS24520 (MUY30_24520) 11028..11417 + 390 Protein_12 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-10 mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..89168 89168
- inside IScluster/Tn - - 2813..16764 13951


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T241643 WP_003026803.1 NZ_CP095167:6424-6906 [Enterobacter roggenkampii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T241643 NZ_LT996890:1683607-1683873 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT241643 WP_003026799.1 NZ_CP095167:6170-6436 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT241643 NZ_LT996890:1683350-1683607 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References